Evidence map›Paper›PMID 35280523›Full record

ArticleBiochemistry and biophysics reports2022

Sequence-specific destabilization of azurin by tetramethylguanidinium-dipeptide ionic liquids.

Roshani Patel, Austin K Clark, Gabriella DeStefano, Isabella DeStefano, Hunter Gogoj, Erin Gray, Aashka Y Patel, Joshua T Hauner, Gregory A Caputo, Timothy D Vaden

Open access · goldAbstract read
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In one paragraph

Article in Biochemistry and biophysics reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Roshani PatelDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Austin K ClarkDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Gabriella DeStefanoDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Isabella DeStefanoDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Hunter GogojDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Erin GrayDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Aashka Y PatelDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Joshua T HaunerDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Gregory A CaputoDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Timothy D VadenDepartment of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, 08028, USA.
Rowan University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The thermal unfolding of the copper redox protein azurin was studied in the presence of four different dipeptide-based ionic liquids (ILs) utilizing tetramethylguanidinium as the cation. The four dipeptides have different sequences including the amino acids Ser and Asp: TMG-AspAsp, TMG-SerSer, TMG-SerAsp, and TMG-AspSer. Thermal unfolding curves generated from temperature-dependent fluorescence spectroscopy experiments showed that TMG-AspAsp and TMG-SerSer have minor destabilizing effects on the protein while TMG-AspSer and TMG-SerAsp strongly destabilize azurin. Red-shifted fluorescence signatures in the 25 °C correlate with the observed protein destabilization in the solutions with TMG-AspSer and TMG-SerAsp. These signals could correspond to interactions between the Asp residue in the dipeptide and the azurin Trp residue in the unfolded state. These results, supported by appropriate control experiments, suggest that dipeptide sequence-specific interactions lead to selective protein destabilization and motivate further studies of TMG-dipeptide ILs.

Indexed as

AzurinDipeptidesIonic liquidsProtein stability

Identifiers

PMID35280523
PMCPMC8907678
OpenAlexW4220932703

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.