ArticlePLoS pathogens2022
Acid stress signals are integrated into the σB-dependent general stress response pathway via the stressosome in the food-borne pathogen Listeria monocytogenes.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 30 citations in OpenAlex.
- Downregulation of motility during stress requires stressosome input inApplied and environmental microbiology · 2026Article
- The glutamate decarboxylase system (GAD2) is activated by osmotic stress in a GadR-dependent manner in Listeria monocytogenes.FEMS microbiology letters · 2026Article
- Growth on corn salad leaves and polystyrene as well as the temperature influence the transcriptome ofCurrent research in food science · 2026Article
- Transcriptome Sequencing Reveals Distinct Stress Response of Listeria monocytogenes 6179 During Logarithmic and Stationary Growth Phases.MicrobiologyOpen · 2025Article
- New insights into the stress response mechanisms of stress-resistantEmerging microbes & infections · 2025Article
- Listeriosis.Nature reviews. Disease primers · 2025Review
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Stress-dependent activation of themBio · 2025Article
- Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis.Nature communications · 2025Article
- Exploring the fate ofFrontiers in microbiology · 2025Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Metabolic reprogramming in the food-borne pathogen Listeria monocytogenes as a critical defence against acid stress.FEMS microbiology letters · 2024Review
- A single point mutation in theFrontiers in microbiology · 2024Article
- The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes - a pilot study.BMC microbiology · 2023Article
- Article
- The Virulence and Infectivity of Listeria monocytogenes Are Not Substantially Altered by Elevated SigB Activity.Infection and immunity · 2023Article
- Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.Communications biology · 2023Article
- TheFrontiers in microbiology · 2023Article
- TheAccess microbiology · 2023Article
- Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The general stress response (GSR) in Listeria monocytogenes plays a critical role in the survival of this pathogen in the host gastrointestinal tract. The GSR is regulated by the alternative sigma factor B (σB), whose role in protection against acid stress is well established. Here, we investigated the involvement of the stressosome, a sensory hub, in transducing low pH signals to induce the GSR. Mild acid shock (15 min at pH 5.0) activated σB and conferred protection against a subsequent lethal pH challenge. A mutant strain where the stressosome subunit RsbR1 was solely present retained the ability to induce σB activity at pH 5.0. The role of stressosome phosphorylation in signal transduction was investigated by mutating the putative phosphorylation sites in the core stressosome proteins RsbR1 (rsbR1-T175A, -T209A, -T241A) and RsbS (rsbS-S56A), or the stressosome kinase RsbT (rsbT-N49A). The rsbS S56A and rsbT N49A mutations abolished the response to low pH. The rsbR1-T209A and rsbR1-T241A mutants displayed constitutive σB activity. Mild acid shock upregulates invasion genes inlAB and stimulates epithelial cell invasion, effects that were abolished in mutants with an inactive or overactive stressosome. Overall, the results show that the stressosome is required for acid-induced activation of σB in L. monocytogenes. Furthermore, they show that RsbR1 can function independently of its paralogues and signal transduction requires RsbT-mediated phosphorylation of RsbS on S56 and RsbR1 on T209 but not T175. These insights shed light on the mechanisms of signal transduction that activate the GSR in L. monocytogenes in response to acidic environments, and highlight the role this sensory process in the early stages of the infectious cycle.
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