ArticleFEMS yeast research2022
Production of abscisic acid in the oleaginous yeast Yarrowia lipolytica.
Article in FEMS yeast research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Engineering Yarrowia Lipolytica for De Novo Biosynthesis of Abscisic Acid.Biotechnology journal · 2026Article
- Engineering Saccharomyces cerevisiae to improve heterologous abscisic acid production.Microbial cell factories · 2026Article
- Article
- Multi-step pathway engineering in probioticMetabolic engineering communications · 2025Article
- Recent advances in engineering non-native microorganisms for poly(3-hydroxybutyrate) production.World journal of microbiology & biotechnology · 2025Review
- Systematic metabolic engineering ofSynthetic and systems biotechnology · 2025Article
- Engineering strategies for enhanced 1', 4'-trans-ABA diol production by Botrytis cinerea.Microbial cell factories · 2024Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Abscisic acid-mediated cytosolic CaJournal of Zhejiang University. Science. B · 2023Article
- Engineering microbes to overproduce natural products as agrochemicals.Synthetic and systems biotechnology · 2023Article
- Engineering ofMetabolic engineering communications · 2022Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abscisic acid (ABA) is a phytohormone with applications in agriculture and human health. ABA can be produced by Botrytis cinerea, a plant pathogenic filamentous fungus. However, the cultivation process is lengthy and strain improvement by genetic engineering is difficult. Therefore, we engineered the oleaginous yeast Yarrowia lipolytica as an alternative host for ABA production. First, we expressed five B. cinerea genes involved in ABA biosynthesis (BcABA1,BcABA2,BcABA3,BcABA4 and BcCPR1) in a Y. lipolytica chassis with optimized mevalonate flux. The strain produced 59.2 mg/L of ABA in small-scale cultivation. Next, we expressed an additional copy of each gene in the strain, but only expression of additional copy of BcABA1 gene increased the ABA titer to 168.5 mg/L. We then integrated additional copies of the mevalonate pathway and ABA biosynthesis encoding genes, and we expressed plant ABA transporters resulting in an improved strain producing 263.5 mg/L and 9.1 mg/g dry cell weight (DCW) ABA. Bioreactor cultivation resulted in a specific yield of 12.8 mg/g DCW ABA; however, surprisingly, the biomass level obtained in bioreactors was only 10.5 g DCW/L, with a lower ABA titer of 133.6 mg/L. While further optimization is needed, this study confirms Y. lipolytica as a potential alternative host for the ABA production.
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