Evidence map›Paper›PMID 35272632›Full record

ArticleBMC microbiology2022

Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor.

Xiaoxia Wang, Licheng Wang, Huaxiong Zhu, Chongzhen Wang, Xiong Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaoxia Wang *Central & Clinical Laboratory of Sanya People's Hospital, Hainan, 572000, Sanya, P. R. China.
Licheng Wang *Central & Clinical Laboratory of Sanya People's Hospital, Hainan, 572000, Sanya, P. R. China.
Huaxiong ZhuCentral & Clinical Laboratory of Sanya People's Hospital, Hainan, 572000, Sanya, P. R. China.
Chongzhen WangCentral & Clinical Laboratory of Sanya People's Hospital, Hainan, 572000, Sanya, P. R. China.
Xiong ZhuCentral & Clinical Laboratory of Sanya People's Hospital, Hainan, 572000, Sanya, P. R. China. zhuxiong6@163.com.
Sanya Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBurkholderia pseudomallei (B. pseudomallei), as a highly pathogenic organism, causes melioidosis, which is a disease of public health importance in many tropical developing countries. Here, we present and validate a novel detection technique, termed multiple cross displacement amplification combined with nanoparticles-based lateral flow biosensor (MCDA-NB), for identifying B. pseudomallei and diagnosing melioidosis.

resultsB. pseudomallei-MCDA targets the TTS1 (Type III secretion system gene cluster 1) to specifically design ten MCDA primers. The nanoparticles-based biosensor (NB) can be combined with B. pseudomallei-MCDA for visually, objective, simply and rapidly reporting reaction results. The optimal amplification conditions of B. pseudomallei-MCDA were 66 °C for 30 min. Assay's sensitivity was 100 fg of genomic DNA in the pure cultures, and the analytical specificity was 100% by the examination of 257 strains, including 228 B. pseudomallei and 29 non-B. pseudomallei. As a result, the whole detection procedure was completed within 50 min, including 15 min for genomic DNA preparation, 30 min for l MCDA reaction, and 2 min for the interpretation of the results visually by biosensor.

conclusionsB. pseudomallei-MCDA assay is a rapid, sensitive and specific method for the detection of B. pseudomallei, and can be used as a potential tool for melioidosis diagnose in basic, field and clinical laboratories.

Indexed as

Biosensing TechniquesBurkholderia pseudomalleiMelioidosisHumansNucleic Acid Amplification TechniquesSensitivity and SpecificityBiosensorBurkholderia pseudomalleiIsothermal amplification techniqueMelioidosisRapid diagnosis

Identifiers

PMID35272632
PMCPMC8908694
OpenAlexW4220779315

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.