Evidence map›Paper›PMID 35272155›Full record

ArticleTalanta2022

Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein.

Patricia Bento da Silva, Jaqueline Rodrigues da Silva, Mosar Corrêa Rodrigues, Julia Augusto Vieira, Ikaro Alves de Andrade, Tatsuya Nagata, Alexandre Silva Santos, Sebastião William da Silva, Marcia Cristina Oliveira da Rocha, Sônia Nair Báo and 16 more

Open access · greenAbstract read
In one paragraph

Article in Talanta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 11 institutions in 5 countries.

Patricia Bento da SilvaLaboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Jaqueline Rodrigues da SilvaLaboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Mosar Corrêa RodriguesLaboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Julia Augusto VieiraLaboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Ikaro Alves de AndradeVirology and Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Tatsuya NagataVirology and Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Alexandre Silva SantosOptical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Sebastião William da SilvaOptical Spectroscopy Laboratory, Institute of Physics, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil.
Marcia Cristina Oliveira da RochaElectron Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasília, Distrito Federal, 70910-900, Brazil.
Sônia Nair BáoElectron Microscopy Laboratory, Cell Biology Department, Institute of Biological Sciences, University of Brasilia, Brasília, Distrito Federal, 70910-900, Brazil.
Pedro M Moraes-VieiraDepartment of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
José Proença-ModenaDepartment of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
Monara K C AngelimDepartment of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
Gabriela Fabiano de SouzaDepartment of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
Stefanie Primon MuraroDepartment of Genetics, Evolution, Microbiology, and Immunology, University of Campinas, Campinas, São Paulo, 13083970, Brazil.
André Luis Branco de BarrosDepartment of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Glêndara Aparecida de Souza MartinsPrograma de Pós-graduação em Ciência e Tecnologia de Alimentos, Federal University of Tocantins, 77001-090, Brazil.
Fátima Ribeiro-DiasLaboratório de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil.
Giovanna MachadoCentro de Tecnologias Estratégicas do Nordeste, Av. Prof. Luiz Freire 01, Recife, Pernambuco, 50740-540, Brazil.
Melissa Regina FesselBioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil.
Ana Marisa Chudzinski-TavassiBioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil.
Célia Machado RonconiDepartamento de Química Inorgânica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro de São João Batista, s/n, 24020-141, Niterói, RJ, Brazil.
Debora GonçalvesSao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, São Paulo, 13560-970, Brazil.
Rui CuriBioindustrial Center, Butantan Institute, Sao Paulo, São Paulo, 05503-900, Brazil; Interdisciplinary Program of Health Sciences, Cruzeiro do Sul University, Sao Paulo, 01311-925, Brazil.
Osvaldo N OliveiraSao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos, São Paulo, 13560-970, Brazil. Electronic address: chu@ifsc.usp.br.
Ricardo Bentes AzevedoLaboratory of Nanobiotechnology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Distrito Federal, 70910-900, Brazil. Electronic address: razevedo@unb.br.
Universidade de Brasília · BRUniversidade Estadual de Campinas (UNICAMP) · BRMorpho (United States) · USInstitute of Physics · PLInstituto Butantan · BRCentro de Tecnologias Estratégicas do Nordeste · BRUniversidade Cruzeiro do Sul · BRUniversidade Federal de Goiás · BRUniversidade Federal de Minas Gerais · BRUniversidade Federal do Tocantins · BRUniversidade Federal Fluminense · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mass testing for the diagnosis of COVID-19 has been hampered in many countries owing to the high cost of genetic material detection. This study reports on a low-cost immunoassay for detecting SARS-CoV-2 within 30 min using dynamic light scattering (DLS). The immunosensor comprises 50-nm gold nanoparticles (AuNPs) functionalized with antibodies against SARS-CoV-2 spike glycoprotein, whose bioconjugation was confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and surface-enhanced Raman scattering spectroscopy (SERS). The specific binding of the bioconjugates to the spike protein led to an increase in bioconjugate size, with a limit of detection (LOD) 5.29 × 10

Indexed as

Biosensing TechniquesCOVID-19Metal NanoparticlesCOVID-19 TestingDynamic Light ScatteringGoldHumansImmunoassaySARS-CoV-2Spike Glycoprotein, CoronavirusViral ProteinsGoldSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Viral ProteinsBioconjugatesImmunosensorsInfluenza virusSARS-CoV-2Spectroscopic techniques

Identifiers

PMID35272155
PMCPMC8895652
OpenAlexW4214887884

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.