Evidence map›Paper›PMID 35271889›Full record

ArticleJournal of virological methods2022

A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.

Mohammad Moradzad, Hasan Soltani, Hamid Salehi, Khaled Rahmani, Dariush Khateri, Mohammad Zaid Rahimi, Diman Az, Shohreh Fakhari

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohammad MoradzadMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran; Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Hasan SoltaniMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Hamid SalehiMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran; Clinical Laboratory Unit, Tohid Hospital, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Khaled RahmaniSocial Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Dariush KhateriMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Mohammad Zaid RahimiDepartment of Medical Genetics and Molecular Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Diman AzMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Shohreh FakhariMolecular Laboratory, Vice Chancellor for Health Affairs, Kurdistan University of Medical Sciences, Sanandaj, Iran; Cancer & Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Electronic address: shohre.fakhari@yahoo.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

By the emergence of SARS CoV-2 variants, many studies were developed to deal with it. The high transmissibility and mortality rate of some variants, in particular developing countries have caused the operation of simple diagnostic tests for genomic surveillance. In this study, we developed two assays of High Resolution Melting (HRM) and Probe-based RT-PCR as simple and inexpensive methods to identify the variants. We screened the mutations of del69-70, E484K, E484Q, D614G, L452R, and T478K in 100 cases from SARS-COV-2 positive patients in Kurdistan- Iran population. In general, the result of the two methods overlapped each other, nevertheless, we suggested HRM results be confirmed with a standard assay (Whole-Genome Sequencing). This work indicated that HRM as the rapid and inexpensive method could identify and categorize the variants of SARS CoV-2 and reduce the costs for carrying out sequencing.

Indexed as

COVID-19SARS-CoV-2HumansIranIraqMutationReverse Transcriptase Polymerase Chain ReactionSpike Glycoprotein, CoronavirusSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2D614G mutationDel69-70 mutationE484K mutationE484Q mutationHRML452R mutationProbe-based RT-PCRSARS CoV-2T478K mutation

Identifiers

PMID35271889
PMCPMC8900876

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.