ArticleInternational journal of molecular sciences2022
LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Metabolomic profiling delineates the role of adenosine in oocyte quality and embryonic development.Cell death & disease · 2026Article
- Non-invasive embryo assessment: Cell-free DNA-based genetic testing and amino acid metabolomics in relation to morphology: A case-control study.International journal of reproductive biomedicine · 2026Article
- Multi-omics biomarkers in female fertility: from oocyte quality to endometrial receptivity and clinical translation.Biomarker research · 2026Review
- Non-invasive assessment of embryo development and ploidy: morphokinetics predict blastulation, and exploratory metabolomics identifies candidate metabolic signatures for euploidy.Frontiers in cell and developmental biology · 2026Article
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- New approaches in the analysis of spent embryo culture media in the IVF process.Archives of gynecology and obstetrics · 2025Article
- Noninvasive Biomarkers of Human Embryo Developmental Potential.International journal of molecular sciences · 2025Review
- The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature.International journal of molecular sciences · 2024Review
- The prospect of artificial intelligence to personalize assisted reproductive technology.NPJ digital medicine · 2024Review
- The association between pregnancy outcomes and frozen-thawed embryo transfer cycles based on D3 cell count in high-quality blastocysts.Frontiers in endocrinology · 2024Article
- Prediction model for day 3 embryo implantation potential based on metabolites in spent embryo culture medium.BMC pregnancy and childbirth · 2023Article
- Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer.Clinical and experimental reproductive medicine · 2022Article
- Modulation of Virulence-Associated Traits inMicroorganisms · 2022Article
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Authors and funding
11 authors.
Funding
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Abstract
In this study we evaluated possible differences in metabolomic profiles of spent embryo culture media (SECM) of human embryos with distinct morphology, karyotype, and implantation outcomes. A total of 153 samples from embryos of patients undergoing in vitro fertilization (IVF) programs were collected and analyzed by HPLC-MS. Metabolomic profiling and statistical analysis revealed clear clustering of day five SECM from embryos with different morphological classes and karyotype. Profiling of day five SECM from embryos with different implantation outcomes showed 241 significantly changed molecular ions in SECM of successfully implanted embryos. Separate analysis of paired SECM samples on days three and five revealed 46 and 29 molecular signatures respectively, significantly differing in culture media of embryos with a successful outcome. Pathway enrichment analysis suggests certain amino acids, vitamins, and lipid metabolic pathways to be crucial for embryo implantation. Differences between embryos with distinct implantation potential are detectable on the third and fifth day of cultivation that may allow the application of culture medium analysis in different transfer protocols for both fresh and cryopreserved embryos. A combination of traditional morphological criteria with metabolic profiling of SECM may increase implantation rates in assisted reproductive technology programs as well as improve our knowledge of the human embryo metabolism in the early stages of development.
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