ArticleDevelopment (Cambridge, England)2022
A constitutively expressed fluorescent ubiquitination-based cell-cycle indicator (FUCCI) in axolotls for studying tissue regeneration.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- A characterization of axolotl digit regeneration: conserved mechanisms, divergent patterning, and a critical role for hedgehog signaling.NPJ Regenerative medicine · 2026Article
- Model recapitulates regenerative limb blastema formation through local softening of the wounded epithelium.bioRxiv : the preprint server for biology · 2026Article
- Neural regulation of H3K27me3 during the induction of patterning competency in regenerating Axolotl limb cells.Communications biology · 2025Article
- Making a new limb out of old cells: exploring endogenous cell reprogramming and its role during limb regeneration.American journal of physiology. Cell physiology · 2024Review
- Integration failure of regenerated limb tissue is associated with incongruencies in positional information in the Mexican axolotl.Frontiers in cell and developmental biology · 2023Article
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Positional Memory in Vertebrate Regeneration: A Century's Insights from the Salamander Limb.Cold Spring Harbor perspectives in biology · 2022Review
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Imaging developmental cell cycles.Biophysical journal · 2021Review
- Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration.eLife · 2021Article
- HDAC Inhibitor Titration of Transcription and Axolotl Tail Regeneration.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Regulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, the axolotl (Ambystoma mexicanum) regenerates missing structures through an accumulation of proliferating cells known as the blastema. To study cell division during blastema growth, we generated a transgenic line of axolotls that ubiquitously expresses a bicistronic version of the fluorescent ubiquitination-based cell-cycle indicator (FUCCI). We demonstrate near-ubiquitous FUCCI expression in developing and adult tissues, and validate these expression patterns with DNA synthesis and mitosis phase markers. We demonstrate the utility of FUCCI for live and whole-mount imaging, showing the predominantly local contribution of cells during limb and tail regeneration. We also show that spinal cord amputation results in increased proliferation at least 5 mm from the site of injury. Finally, we use multimodal staining to provide cell type information for cycling cells by combining fluorescence in situ hybridization, EdU click-chemistry and immunohistochemistry on a single FUCCI tissue section. This new line of animals will be useful for studying cell cycle dynamics using in situ endpoint assays and in vivo imaging in developing and regenerating animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.