Evidence map›Paper›PMID 35265868›Full record

ArticleCell reports. Physical science2022

Complete inhibition of a polyol nucleation by a micromolar biopolymer additive.

Xin Wen, Sen Wang, Robert Ramji, Luke O Butler, Yelena Bagdagulyan, Audrey Kishishita, James A Golen, Arnold L Rheingold, Soo-Kyung Kim, William A Goddard and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Physical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. TiMikrochimica acta · 2025
    Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Xin WenDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA 90032, USA.
Sen WangDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA 90032, USA.
Robert RamjiATLAS Materials Physics Laboratory, Department of NanoEngineering and Chemical Engineering, University of California, San Diego, La Jolla, CA 92093, USA.
Luke O ButlerATLAS Materials Physics Laboratory, Department of NanoEngineering and Chemical Engineering, University of California, San Diego, La Jolla, CA 92093, USA.
Yelena BagdagulyanDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA 90032, USA.
Audrey KishishitaDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA 90032, USA.
James A GolenUniversity of California San Diego Materials Research Science and Engineering Center, University of California, San Diego, La Jolla, CA 92093, USA.
Arnold L RheingoldUniversity of California San Diego Materials Research Science and Engineering Center, University of California, San Diego, La Jolla, CA 92093, USA.
Soo-Kyung KimMaterials and Process Simulation Center, California Institute of Technology, Pasadena, CA 91125, USA.
William A GoddardMaterials and Process Simulation Center, California Institute of Technology, Pasadena, CA 91125, USA.
Tod A PascalATLAS Materials Physics Laboratory, Department of NanoEngineering and Chemical Engineering, University of California, San Diego, La Jolla, CA 92093, USA.
University of California San Diego · USCalifornia State University Los Angeles · USCalifornia Institute of Technology · US

Funding

UNDERGRADUATE STUDENT TRAINING IN ACADEMIC RESEARCHT34GM008228 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI TUNSTAD, LINDA M. · 1986 to 2022
$13.3M
IDENTIFICATION AND MOLECULAR BASIS FOR EFFICIENT ANTIFREEZE PROTIEN ENHANCERSSC3GM086249 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI WEN, XIN · 2009 to 2023
$1.7M
Time-of-Flight Mass Spectrometer for Synthesis Supporting Biomedical ResearchS10RR025631 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HOUK, KENDALL N · 2009 to 2009
$328k
NCRR NIH HHS S10 RR025631NIGMS NIH HHS SC3 GM086249NIGMS NIH HHS T34 GM008228
6 · The paper itself

Abstract

Preventing spontaneous crystallization of supersaturated solutions by additives is of critical interest to successful process design and implementation, with numerous applications in chemical, pharmaceutical, medical, pigment, and food industries, but challenges remain in laboratory and industry settings and fundamental understanding is lacking. When copresented with antifreeze proteins (AFPs), otherwise spontaneously crystallizing osmolytes are maintained at high supersaturations for months in over-wintering organisms. Thus, we here explore the inhibition phenomenon by AFPs, using persistent crystallization of a common sugar alcohol, D-mannitol, as a case study. We report experimentally that DAFP1, an insect AFP, completely inhibits D-mannitol nucleation. Computer simulations reveal a new mechanism for crystallization inhibition where the population of the crystal-forming conformers are selectively bound and randomized in solution by hydrogen bonding to the protein surface. These results highlight the advantages of using natural polymers to address crystallization inhibition challenges and suggest new strategies in controlling the nucleation processes.

Identifiers

PMID35265868
PMCPMC8903182
OpenAlexW4206732816

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.