ReviewFrontiers in cell and developmental biology2022
Toward Best Practices for Controlling Mammalian Cell Culture Environments.
Review in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- IncuSense: IoT-Enabled Wireless Long-Term Multiparametric Cell Culture Incubator Monitoring System.Annals of biomedical engineering · 2026Article
- Functional In Vitro Model of the Canine Corpus Luteum: Isolation, Culture and Characterization of Steroidogenically Active Luteal Cells.Biomedicines · 2026Article
- A human relevant in vitro alveolar epithelial barrier model to assess inhaled pollutant hazard.Scientific reports · 2025Article
- Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.PLoS biology · 2025Article
- Shiga toxin targets the podocyte causing hemolytic uremic syndrome through endothelial complement activation.Med (New York, N.Y.) · 2023Article
- Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe.Journal of the American Society for Mass Spectrometry · 2023Article
- Article
- Transcriptional Response in Human Jurkat T Lymphocytes to a near Physiological Hypergravity Environment and to One Common in Routine Cell Culture Protocols.International journal of molecular sciences · 2023Article
- Article
- In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture.Communications biology · 2022Article
- Applying the Cytocentric Principles to Regenerative Medicine for Reproducibility.Current stem cell reports · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The characterization, control, and reporting of environmental conditions in mammalian cell cultures is fundamental to ensure physiological relevance and reproducibility in basic and preclinical biomedical research. The potential issue of environment instability in routine cell cultures in affecting biomedical experiments was identified many decades ago. Despite existing evidence showing variable environmental conditions can affect a suite of cellular responses and key experimental readouts, the underreporting of critical parameters affecting cell culture environments in published experiments remains a serious problem. Here, we outline the main sources of potential problems, improved guidelines for reporting, and deliver recommendations to facilitate improved culture-system based research. Addressing the lack of attention paid to culture environments is critical to improve the reproducibility and translation of preclinical research, but constitutes only an initial step towards enhancing the relevance of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.