Evidence map›Paper›PMID 35265138›Full record

ArticleJournal of tropical medicine2022

Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay for a Rapid and Sensitive Detection of Cystic Echinococcosis in Livestock in Kenya.

Nasser Arreh Badoul, John Kagira, Florence Ng'ong'a, Hunduma Dinka

Open access · goldAbstract read
In one paragraph

Article in Journal of tropical medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Nasser Arreh BadoulDepartment of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation, Juja, Kenya.ORCID https://orcid.org/0000-0002-9087-9850
John KagiraDepartment of Animal Sciences, School of Natural Resources and Animal Sciences, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.ORCID https://orcid.org/0000-0001-5513-2113
Florence Ng'ong'aDepartment of Biochemistry, School of Biomedical Sciences, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya.ORCID https://orcid.org/0000-0002-9953-1746
Hunduma DinkaDepartment of Applied Biology, School of Applied Natural Sciences, Adama Science and Technology University, Adama, Ethiopia.ORCID https://orcid.org/0000-0002-3498-7689
Jomo Kenyatta University of Agriculture and Technology · KEAdama Science and Technology University · ETPan African University Institute for Basic Sciences, Technology and Innovation

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Cystic echinococcosis is a zoonotic disease caused by the metacestode stage of Results: In this study, a specific primer set and FITC-labeled probe targeting the conserved region of the NADH-1 gene were designed. The LAMP reaction was performed at 60°C for 40 minutes, and the amplification products were successfully visualized by LFD strips. The analytical sensitivity of LAMP-LFD was determined using 10-fold serial dilutions of Conclusion: Our results indicated that the developed LAMP-LFD technique was more sensitive than the nested PCR assay, rapid, and easy to perform with a simple visual detection of products. Therefore, it could be an important point-of-care diagnostic tool for cystic echinococcosis.

Identifiers

PMID35265138
PMCPMC8898851
OpenAlexW4214746956

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.