ArticleScientific reports2022
Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Manual curation for improved genome annotation of the functionally extinct northern white rhinoceros (Ceratotherium simum cottoni).PloS one · 2026Article
- De-extinction of the Northern white rhinoceros (Ceratotherium simum cottoni).The Journal of reproduction and development · 2026Review
- Article
- Establishment, Characterization, and Cryopreservation of Feather Follicle Fibroblast Lines From Hyacinth Macaw (Anodorhynchus hyacinthinus).Cell biology international · 2025Article
- Genomic map of the functionally extinct northern white rhinoceros (Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dissecting the development of the early lineages and primordial germ cells in the bovine embryo.Animal reproduction · 2025Article
- Reporter Alleles in hiPSCs: Visual Cues on Development and Disease.International journal of molecular sciences · 2024Review
- A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material.Animals : an open access journal from MDPI · 2024Article
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species.Scientific reports · 2024Article
- 40 'wild' years: the current reality and future potential of assisted reproductive technologies in wildlife species.Animal reproduction · 2024Review
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- 40 years of AETE: the contribution of scientists and practitioners to the progress of reproductive biotechnologies in Europe.Animal reproduction · 2024Review
- Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing.Stem cell reports · 2023Article
- In vitro fertilization program in white rhinoceros.Reproduction (Cambridge, England) · 2023Article
- Generation of induced pluripotent stem cells from Bornean orangutans.Frontiers in cell and developmental biology · 2023Article
- Robust induction of primordial germ cells of white rhinoceros on the brink of extinction.Science advances · 2022Article
Corrections and comments
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Authors and funding
22 authors at 10 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The northern white rhinoceros (NWR) is probably the earth's most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.
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