ArticleParasites & vectors2022
Interactions between Blastocystis subtype ST4 and gut microbiota in vitro.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Global Prevalence and Subtype Distribution of Blastocystis sp. in Rodent Populations: A Systematic Review and Meta-Analysis.Veterinary medicine and science · 2025Pooled it
- Gut protozoa of wild rodents - a meta-analysis.Parasitology · 2024Pooled it
- Molecular Detection ofVeterinary sciences · 2026Article
- Blastocystis, an Enigmatic Parasite.Acta parasitologica · 2026Review
- Interactions between bacterial microbiota and protozoan parasites: an intimate relationship.Archives of microbiology · 2026Review
- Interpreting probiotic and combination-therapy studies in Blastocystis.Gut pathogens · 2026Article
- Lactobacillus probiotics potential in Blastocystis infection: in vitro and in vivo studies.Gut pathogens · 2025Article
- Article
- Comprehensive Tools for Culturing Blastocystis: A Standardized Resource for Research and Diagnostics.Current protocols · 2025Article
- Impact of Blastocystis carriage and colonization intensity on gut microbiota composition in a non-westernized rural population from Colombia.PLoS neglected tropical diseases · 2025Article
- Decoding Blastocystis-Driven Mechanisms in Gut Microbiota and Host Metabolism.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Evidence of pathogens associated with travelers' diarrhea in Thailand: a systematic review.Tropical diseases, travel medicine and vaccines · 2025Article
- Article
- Article
- Microbial and metabolic signatures amongCurrent research in parasitology & vector-borne diseases · 2025Article
- Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model.Frontiers in cellular and infection microbiology · 2025Article
- Genetic diversity of single-celled microorganismCurrent research in microbial sciences · 2025Article
- New Haplotypes of Blastocystis sp. Identified in Faeces from Various Animal Groups in Algeria.Acta parasitologica · 2024Article
- Unveiling Blastocystis epidemiology in Morocco: subtype diversity among clinical patients with and without gastrointestinal manifestations in the Meknes region.Parasitology research · 2024Article
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBlastocystis ST4 is a common protistan parasite of the gastrointestinal tract of humans and a wide range of animals. While it has been suggested that colonization with ST4 is associated with healthy gut microbiota, how ST4 influences the gut microbiota remains poorly studied. This study aimed to examine the interactions between ST4 and several intestinal bacteria using in vitro co-culture systems, and to further investigate the mechanism of interaction and its effect on the epithelial barrier integrity of HT-29 cells.
methodsSeven intestinal bacteria Bacteroides fragilis, Bifidobacterium longum, Bacillus subtilis, Bacteroides vulgatus, Escherichia coli, Enterococcus faecalis, and Lactobacillus brevis were co-cultured with Blastocystis ST4 in vitro. Flow cytometry and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to determine the role of reactive oxygen species (ROS) and bacteria oxidoreductase genes, respectively, in response to Blastocystis co-incubation. Transepithelial electrical resistance (TEER) and flux assays were performed to assess the effect of microbiota representatives on the integrity of the intestinal epithelial barrier.
resultsCo-incubation with Blastocystis ST4 showed a beneficial influence on most intestinal bacteria, while ST4 significantly inhibited the growth of B. vulgatus, a common pathogen in the genus Bacteroides. The decrease in B. vulgatus when co-incubated with Blastocystis ST4 was associated with high levels of ROS and the upregulation of oxidative stress-related genes. Furthermore, co-incubation with Blastocystis ST4 was able to protect the intestinal epithelial barrier from damage by B. vulgatus.
conclusionsThis study demonstrated, for the first time, that Blastocystis ST4 has beneficial effects on intestinal commensal bacteria in vitro, and can inhibit the growth of pathogenic B. vulgatus. Combined with previous microbiome research on ST4, our data suggest that ST4 may be a beneficial commensal.
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