Evidence map›Paper›PMID 35260160›Full record

ArticleMicrobial cell factories2022

A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation.

Kotaro Mori, Kaho Fukui, Ryotaro Amatsu, Shu Ishikawa, Valeria Verrone, Anil Wipat, Wilfried J J Meijer, Ken-Ichi Yoshida

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In one paragraph

Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Atypical catabolite repression ofMicrobiology (Reading, England) · 2026
    Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kotaro MoriDepartment of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan.
Kaho FukuiDepartment of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan.
Ryotaro AmatsuDepartment of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan.
Shu IshikawaDepartment of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan.
Valeria VerroneSchool of Computing, Newcastle University, 1 Science Square, Science Central, Newcastle upon Tyne, NE4 5TG, UK.
Anil WipatSchool of Computing, Newcastle University, 1 Science Square, Science Central, Newcastle upon Tyne, NE4 5TG, UK.
Wilfried J J MeijerCentro de Biología Molecular 'Severo Ochoa', CSIC-UAM Universidad Autónoma Madrid, Canto Blanco, 28049, Madrid, Spain.
Ken-Ichi YoshidaDepartment of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 5801, Japan. kenyoshi@kobe-u.ac.jp.

Funding

Japan Society for the Promotion of Science 18H02128Spanish Government PID2019 108778GB C21 (AEI/FEDER, EU)
6 · The paper itself

Abstract

backgroundGeobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis.

resultsIn this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation.

conclusionsThis transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus.

Indexed as

Bacillus subtilisGeobacillusChromosomesPlasmidsBacillus subtilisConjugationGeobacillus kaustophilusTransformation

Identifiers

PMID35260160
PMCPMC8903633

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.