ArticleMethods in molecular biology (Clifton, N.J.)2022
Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forage and turf grasses are widely grown and contribute significantly to sustainable agriculture. This chapter describes a protocol for protoplast transformation and plant regeneration for major forage and turf grass species, including tall fescue, red fescue, meadow fescue, perennial ryegrass, and Italian ryegrass. Embryogenic calli induced from caryopsis were used to establish embryogenic cell suspension cultures. Protoplasts were isolated from embryogenic suspension cultures and used for direct gene transfer. Chimeric genes were introduced into protoplasts by polyethylene glycol treatment. Upon selection with antibiotics or herbicide, resistant calli were obtained and transgenic plants were regenerated from these calli.
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Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.