Evidence map›Paper›PMID 35258234›Full record

ArticleClinical chemistry and laboratory medicine2022

Identification of contagious SARS-CoV-2 infected individuals by Roche's Rapid Antigen Test.

Harald H Kessler, Florian Prüller, Melina Hardt, Evelyn Stelzl, Esther Föderl-Höbenreich, Sabine Pailer, Andreas Lueger, Philipp Kreuzer, Kurt Zatloukal, Markus Herrmann

Open access · bronzeAbstract read
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In one paragraph

Article in Clinical chemistry and laboratory medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Harald H KesslerMolecular Diagnostics Laboratory, Diagnostic and Research Institute of Hygiene,Microbiology, and Environmental Medicine, Medical University of Graz, Graz, Austria.
Florian PrüllerClinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
Melina HardtDiagnostic- and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of Graz, Graz, Austria.
Evelyn StelzlMolecular Diagnostics Laboratory, Diagnostic and Research Institute of Hygiene,Microbiology, and Environmental Medicine, Medical University of Graz, Graz, Austria.
Esther Föderl-HöbenreichDiagnostic- and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of Graz, Graz, Austria.
Sabine PailerClinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
Andreas LuegerDivision of Emergency Medicine, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
Philipp KreuzerDivision of Emergency Medicine, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
Kurt ZatloukalDiagnostic- and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of Graz, Graz, Austria.
Markus HerrmannMolecular Diagnostics Laboratory, Diagnostic and Research Institute of Hygiene,Microbiology, and Environmental Medicine, Medical University of Graz, Graz, Austria.
Medical University of Graz · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesRapid antigen tests (RAT) can provide valuable information on the presence or absence SARS-CoV-2 within 15 min without the need of a laboratory. The analytical and diagnostic characteristics of available RATs has led to the question whether they can safely distinguish between infectious and non-infectious patients in an acute care setting.

methodsThree nasopharyngeal swabs for the analysis by RAT, reverse transcriptase real time polymerase chain reaction (RT-qPCR), and a cell culture based infection assay were collected from 67 patients that presented to the emergency department of the University Hospital of Graz (Austria). The first swab was used for on-site RAT testing in the emergency department using the Roche SARS-CoV-2 RAT. The second swab was sent to the central laboratory of the hospital for RT-qPCR with two independent methods (Cepheid Xpert

resultsThe RATs performed from independent samples showed substantial agreement (Cohen's-kappa: 0.73, p<0.001). All patients with a positive RAT had positive RT-qPCR with cycle threshold (ct) values <25. Fifteen out of 55 RAT-negative samples were RT-qPCR positive with ct values between 25 and 40. The inoculation of cell cultures with RT-qPCR negative swabs and RT-qPCR positive swabs with ct values >25 did not induce cytopathic effects that were related to SARS-CoV-2. The infection assays from four RAT-negative patients showed cytopathic effects that were induced by other pathogens.

conclusionsThe SARS-CoV-2 RAT from Roche Diagnostics is a valuable tool for managing symptomatic patients. RAT-negative patients may be regarded as non-contagious.

Indexed as

COVID-19SARS-CoV-2COVID-19 TestingHumansSensitivity and SpecificitySpecimen Handlingantibody assaysCOVID-19IgGIgMnucleocapsideSARS-CoV-2spike

Identifiers

PMID35258234
OpenAlexW4221080450

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.