Evidence map›Paper›PMID 35257116›Full record

ArticleSTAR protocols2022

High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy.

Nicolas Grillet

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Nicolas GrilletDepartment of Otolaryngology-Head and Neck Surgery, School of Medicine, Stanford University, 240 Pasteur Drive, Biomedical Innovation Building, Room 1654, Stanford, CA 94305, USA.
Stanford University · US

Funding

Function of LOXHD1 in mechanosensory hair cellsR01DC016409 · NIDCD · STANFORD UNIVERSITY · PI GRILLET, NICOLAS · 2018 to 2022
$2.1M
High-resolution localization of the hair cell mechanotransduction channel components by immunogold-scanning electronic microscopyR21DC019457 · NIDCD · STANFORD UNIVERSITY · PI GRILLET, NICOLAS · 2021 to 2022
$435k
NIDCD NIH HHS R01 DC016409NIDCD NIH HHS R21 DC019457
6 · The paper itself

Abstract

Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).

Indexed as

Ear, InnerHair Cells, AuditoryAnimalsHairMechanical PhenomenaMiceMicroscopy, Electron, ScanningCell BiologyMicroscopyNeuroscience

Identifiers

PMID35257116
PMCPMC8897606
OpenAlexW4214909350

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.