Evidence map›Paper›PMID 35257114›Full record

ArticleSTAR protocols2022

GBS-MeDIP: A protocol for parallel identification of genetic and epigenetic variation in the same reduced fraction of genomes across individuals.

Shiva Rezaei, Julia Uffenorde, Oliver Gimm, Mohammad Ali Hosseinpour Feizi, Stefan Miemczyk, Luiz Lehmann Coutinho, Per Jensen, Carlos Guerrero-Bosagna, Fábio Pértille

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Chicken Erythrocyte: Epigenomic Regulation of Gene Activity.International journal of molecular sciences · 2023
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Shiva RezaeiDepartment of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 5166616471, Iran.
Julia UffenordeDepartment of Surgery and Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
Oliver GimmDepartment of Surgery and Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
Mohammad Ali Hosseinpour FeiziDepartment of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 5166616471, Iran.
Stefan MiemczykAvian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
Luiz Lehmann CoutinhoEnvironmental Toxicology Program, Department of Organismal Biology, Uppsala University, 752 36 Uppsala, Sweden.
Per JensenAvian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
Carlos Guerrero-BosagnaAvian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
Fábio PértilleDepartment of Surgery and Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
Linköping University · SEUppsala University · SEUniversity of Tabriz · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input for the MeDIP. The GBS-MeDIP is particularly suitable to identify genetic and methylomic biomarkers when resources for whole genome interrogation are lacking.

Indexed as

DNADNA MethylationEpigenesis, GeneticGenotypeHumansImmunoprecipitationDNACancerClinical ProtocolGenomicsHigh Throughput ScreeningMolecular BiologySequencing

Identifiers

PMID35257114
PMCPMC8897576
OpenAlexW4214812113

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.