ArticleCommunications biology2022
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- Rapid turnover of sex-determining architecture in invasive mussels.bioRxiv : the preprint server for biology · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Leveraging sex determination systems for genetic biocontrol of dipteran pests.Current opinion in insect science · 2026Review
- Advances in male sex separation for the support of mosquito control programs.Frontiers in insect science · 2026Review
- Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes.Nature communications · 2025Article
- Mosquito sex determination: recent advances and applications.Current opinion in insect science · 2025Review
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Evaluation of ebony as a potential selectable marker for genetic sexing in Aedes aegypti.Parasites & vectors · 2025Article
- Development of the Incompatible Insect Technique targetingApplied and environmental microbiology · 2025Article
- Combining transinfectedProceedings. Biological sciences · 2024Article
- On the Origin and Evolution of the Mosquito Male-determining Factor Nix.Molecular biology and evolution · 2024Article
- Efficient sex separation by exploiting differential alternative splicing of a dominant marker in Aedes aegypti.PLoS genetics · 2023Article
- Article
- Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control.Communications biology · 2023Article
- A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.Nature communications · 2023Article
- Article
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
- Partial masculinization of Aedes aegypti females by conditional expression of Nix.PLoS neglected tropical diseases · 2022Article
- Natural and Engineered Sex Ratio Distortion in Insects.Frontiers in ecology and evolution · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control.
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