ArticleScientific reports2022
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Absolute voltage mapping using dynamic photocycle control.bioRxiv : the preprint server for biology · 2026Article
- Bright calcium-modulated bioluminescent indicators for activity imaging and red photon-assisted synaptic transmission.bioRxiv : the preprint server for biology · 2026Article
- Two-photon characterisation of long-Stokes-shift dye ATTO 490LS for single-laser multicolour imaging.microPublication biology · 2026Article
- Long-Stokes-Shift mScarlet3 as a Structural Marker for Two-Photon Imaging.microPublication biology · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Positive-going hybrid indicators for voltage imaging in excitable cells and tissues.Science advances · 2025Article
- Imaging sensory transmission and neuronal plasticity in primary sensory neurons with a positively tuned voltage indicator.Nature communications · 2025Article
- Article
- Virtual blebbistatin: A robust and rapid software approach to motion artifact removal in optical mapping of cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.International journal of molecular sciences · 2023Review
- A fully-automated low-cost cardiac monolayer optical mapping robot.Frontiers in cardiovascular medicine · 2023Article
- Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.Cell · 2022Article
- Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice.Science translational medicine · 2022Article
- Eavesdropping wires: Recording activity in axons using genetically encoded calcium indicators.Journal of neuroscience methods · 2021Review
- Multivalent designed proteins protect against SARS-CoV-2 variants of concern.bioRxiv : the preprint server for biology · 2021Article
- Article
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Authors and funding
10 authors.
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Abstract
A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in the presence of sample motion. We performed combinatorial multi-site mutagenesis on a cyan-excitable red fluorescent protein to create the bright and monomeric mCyRFP3, which proved to be uniquely non-perturbing when fused to the GEVI ASAP3. The green/red ratio from ASAP3-mCyRFP3 (ASAP3-R3) reported voltage while correcting for motion artifacts, allowing the visualization of membrane voltage changes in contracting cardiomyocytes and throughout the cell cycle of motile cells.
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