ArticleAnimal microbiome2022
The microbiome of common bedding materials before and after use on commercial dairy farms.
Article in Animal microbiome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Variations in cow milk and teat skin microbiota across the lactation cycle with intramammary cephalosporin use at dry-off.Applied and environmental microbiology · 2026Article
- Host-microbiome interplay supports heat stress resilience in zebu calves.Animal microbiome · 2026Article
- Insights into spatial dynamics of the microbiome and resistome across the conventional and organic dairy farms.PloS one · 2026Article
- Characterization of Bacterial Communities in Air and Bedding Materials of Intensive Donkey Farms During Summer.Microorganisms · 2025Article
- Efficacy of lime-based conditioner against environmental udder pathogens in different bedding materials.Veterinary and animal science · 2025Article
- Cluster ofFrontiers in veterinary science · 2025Article
- Modeling Mastitis Risk Management Effects on Dairy Milk Yield and Global Warming Potential.Animals : an open access journal from MDPI · 2024Article
- The gut microbiome and resistome of yellow perch (One health (Amsterdam, Netherlands) · 2024Article
- Conditioner application improves bedding quality and bacterial composition with potential beneficial impacts for dairy cow's health.Microbiology spectrum · 2024Article
- Tracking the microbial communities from the farm to the processing facility of a washed-rind cheese operation.Frontiers in microbiology · 2024Article
- Mock community as an in situ positive control for amplicon sequencing of microbiotas from the same ecosystem.Scientific reports · 2023Article
- Effect of Husbandry Practices on the Fecal Microbiota of C57BL/6J Breeding Colonies Housed in 2 Different Barrier Facilities in the Same Institution.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Transmission of antimicrobial resistance (AMR) during animal transport.EFSA journal. European Food Safety Authority · 2022Article
- Characterization of rumen, fecal, and milk microbiota in lactating dairy cows.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundBovine mastitis is one of the most economically important diseases affecting dairy cows. The choice of bedding material has been identified as an important risk factor contributing to the development of mastitis. However, few reports examine both the culturable and nonculturable microbial composition of commonly used bedding materials, i.e., the microbiome. Given the prevalence of nonculturable microbes in most environments, this information could be an important step to understanding whether and how the bedding microbiome acts as a risk factor for mastitis. Therefore, our objective was to characterize the microbiome composition and diversity of bedding material microbiomes, before and after use.
methodsWe collected 88 bedding samples from 44 dairy farms in the U.S. Unused (from storage pile) and used (out of stalls) bedding materials were collected from four bedding types: new sand (NSA), recycled manure solids (RMS), organic non-manure (ON) and recycled sand (RSA). Samples were analyzed using 16S rRNA sequencing of the V3-V4 region.
resultsThe overall composition as well as the counts of several microbial taxa differed between bedding types, with Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes dominating across all types. Used bedding contained a significantly different microbial composition than unused bedding, but the magnitude of this difference varied by bedding type, with RMS bedding exhibiting the smallest difference. In addition, positive correlations were observed between 16S rRNA sequence counts of potential mastitis pathogens (bacterial genera) and corresponding bedding bacterial culture data.
conclusionOur results strengthen the role of bedding as a potential source of mastitis pathogens. The consistent shift in the microbiome of all bedding types that occurred during use by dairy cows deserves further investigation to understand whether this shift promotes pathogen colonization and/or persistence, or whether it can differentially impact udder health outcomes. Future studies of bedding and udder health may be strengthened by including a microbiome component to the study design.
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