ArticleCell & bioscience2022
Generation of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver.
Article in Cell & bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Epigenetic Drift and the Generational Limit of Serial Somatic Cell Nuclear Transfer in Pigs.Animals : an open access journal from MDPI · 2026Review
- Evolutionary insights and structural characterization guide the development of RAG1/RAG2-deficient swine models for immunological research.Frontiers in immunology · 2026Article
- Achieving scalable expansion of therapeutic porcine hepatocytes in vivo through serial transplantation.Animal models and experimental medicine · 2025Article
- Homozygous editing of multiple genes for accelerated generation of xenotransplantation pigs.Genome research · 2025Article
- Cell therapy for liver disorders: past, present and future.Nature reviews. Gastroenterology & hepatology · 2025Review
- Genetic engineering drives the breakthrough of pig models in liver disease research.Liver research (Beijing, China) · 2024Review
- Elimination of GGTA1, CMAH, β4GalNT2 and CIITA genes in pigs compromises human versus pig xenogeneic immune reactions.Animal models and experimental medicine · 2024Article
- Generation and transcriptomic characterization of MIR137 knockout miniature pig model for neurodevelopmental disorders.Cell & bioscience · 2024Article
- Functional mouse hepatocytes derived from interspecies chimeric livers effectively mitigate chronic liver fibrosis.Stem cell reports · 2024Article
- Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos.Frontiers in veterinary science · 2024Article
- Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress.Animals : an open access journal from MDPI · 2023Article
- Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers.Cells · 2023Review
- Development ofFrontiers in immunology · 2022Article
- Porcine Wound Models: The Gold Standard for Translational Research in Cutaneous Healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyReview
Corrections and comments
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Authors and funding
27 authors at 8 institutions in 1 country.
Funding
Abstract
backgroundMice with humanized livers are important models to study drug toxicology testing, development of hepatitis virus treatments, and hepatocyte transplantation therapy. However, the huge difference between mouse and human in size and anatomy limited the application of humanized mice in investigating human diseases. Therefore, it is urgent to construct humanized livers in pigs to precisely investigate hepatocyte regeneration and human hepatocyte therapy. CRISPR/Cas9 system and somatic cell cloning technology were used to generate two pig models with FAH deficiency and exhibiting severe immunodeficiency (FAH/RAG1 and FAH/RAG1/IL2RG deficiency). Human primary hepatocytes were then successfully transplanted into the FG pig model and constructed two pigs with human liver.
resultsThe constructed FAH/RAG1/IL2RG triple-knockout pig models were characterized by chronic liver injury and severe immunodeficiency. Importantly, the FG pigs transplanted with primary human hepatocytes produced human albumin in a time dependent manner as early as 1 week after transplantation. Furthermore, the colonization of human hepatocytes was confirmed by immunochemistry staining.
conclusionsWe successfully generated pig models with severe immunodeficiency that could construct human liver tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.