ArticleBMC genomics2022
RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats.
Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- A Functionally Conserved Enhancer is Critical for PGC1A Expression and Thermogenesis in Brown Fat.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Developmental transition of brown adipose tissue from a thermogenic to a lipogenic phenotype in neonatal goats revealed by single-nucleus transcriptomics.Zoological research · 2026Article
- Brown adipose tissue in ruminants: development, thermogenic function, and nutritional regulation.Frontiers in veterinary science · 2026Review
- The Role of miR-326-3p in Regulating Differentiation and Thermogenesis Genes in Goat Brown Adipocytes.Genes · 2025Article
- Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus.BMC genomics · 2025Article
- White Adipocyte Stem Cell Expansion Through Infant Formula Feeding: New Insights into Epigenetic Programming Explaining the Early Protein Hypothesis of Obesity.International journal of molecular sciences · 2025Review
- Short-term heat exposure affects thermogenesis and mitophagy in goat brown adipocytes.BMC genomics · 2025Article
- Negative energy balance by feed deprivation affects the adipose miRNome in the lactating goat.Journal of animal science · 2025Article
- Identification of key genes regulating brown adipose tissue thermogenesis in goat kids (Frontiers in veterinary science · 2025Article
- Current status of transcriptome sequencing technology in ruminants.Frontiers in veterinary science · 2025Review
- Pulling the trigger: Noncoding RNAs in white adipose tissue browning.Reviews in endocrine & metabolic disorders · 2024Review
- A role for miRNAs in the regulation of brown adipose tissue whitening in goats (Capra Hircus).Journal of animal science · 2024Article
- Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats.International journal of molecular sciences · 2023Article
- A Novel LncRNA MSTRG.310246.1 Promotes Differentiation and Thermogenesis in Goat Brown Adipocytes.Genes · 2023Article
- Article
- Cold exposure induces lipid dynamics and thermogenesis in brown adipose tissue of goats.BMC genomics · 2022Article
- Regulation of Adipose Thermogenesis and its Critical Role in Glucose and Lipid Metabolism.International journal of biological sciences · 2022Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are a family of short non-coding RNA molecules and play important roles in various biological processes. However, knowledge of the expression profiles and function of miRNAs on the regulation of brown adipose tissue (BAT) thermogenesis remains largely unknown.
resultsIn this study, we found that brown adipose tissue (BAT) existed within the perirenal fat at 1 day after birth (D1) and transferred into white adipose tissue (WAT) at 30 days after birth (D30) by UCP1 protein expression and immunohistochemistry analysis. After that, we performed RNA sequencing on six libraries of goat BAT and WAT. A total of 238 known miRNAs and 1834 goat novel miRNAs were identified. Moreover, 395 differentially expressed miRNAs including 167 up-regulated and 228 down-regulated miRNAs were obtained in BAT. For the known BAT enriched miRNA, 30 miRNAs were enriched in goat BAT but not in mouse BAT. In addition, miR-433 was enriched in goat BAT but not in mouse BAT. Gain- and loss-of-function experiments reveal that miR-433 reduced the lipid accumulation of brown adipocytes and decreased the expression of BAT marker and mitochondrial related genes. However, miR-433 had no effect on lipid accumulation and thermogenesis in white adipocytes. In addition, miR-433 inhibited the expression of MAPK8 by targeting to the 3'UTR of MAPK8 gene. These data demonstrate that miR-433 acts as a negative regulator in controlling brown adipocytes differentiation and thermogenesis.
conclusionThe present study provides a detailed miRNAs expression landscape in BAT and WAT. Furthermore, we found that miR-433, which was highly expressed on BAT had a negative regulatory function on the thermogenesis and adipogenesis in goat brown adipocytes. This study provides evidence for understanding the role of miRNAs in regulating BAT thermogenesis and energy expenditure in goats.
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