ArticleThe Journal of cell biology1986
Myosin at the apical pole of ciliated epithelial cells as revealed by a monoclonal antibody.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- Plakophilin 3 Is Involved in Basal Body Docking in Multiciliated Cells.International journal of molecular sciences · 2025Article
- Cell polarity: having and making sense of direction-on the evolutionary significance of the primary cilium/centrosome organ in Metazoa.Open biology · 2018Review
- Addressing the Functional Determinants of FAK during Ciliogenesis in Multiciliated Cells.The Journal of biological chemistry · 2017Article
- Multiciliated Cells in Animals.Cold Spring Harbor perspectives in biology · 2016Review
- Loss of MACF1 Abolishes Ciliogenesis and Disrupts Apicobasal Polarity Establishment in the Retina.Cell reports · 2016Article
- Centrosome positioning in non-dividing cells.Protoplasma · 2016Review
- Leading-process actomyosin coordinates organelle positioning and adhesion receptor dynamics in radially migrating cerebellar granule neurons.Neural development · 2014Article
- The fate of the primary cilium during myofibroblast transition.Molecular biology of the cell · 2014Article
- Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton.Journal of cell science · 2009Article
- Nucleokinesis in tangentially migrating neurons comprises two alternating phases: forward migration of the Golgi/centrosome associated with centrosome splitting and myosin contraction at the rear.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells.The Journal of cell biology · 1998Article
- Microinjection of the catalytic fragment of myosin light chain kinase into dividing cells: effects on mitosis and cytokinesis.The Journal of cell biology · 1991Article
- Cytochalasin D inhibits basal body migration and ciliary elongation in quail oviduct epithelium.Cell and tissue research · 1990Article
- The cortical microfilament system of lymphoblasts displays a periodic oscillatory activity in the absence of microtubules: implications for cell polarity.The Journal of cell biology · 1989Article
- Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.Infection and immunity · 1987Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A monoclonal antibody (CC-212), obtained in a fusion experiment in which basal bodies from quail oviduct were used as immunogen, has been shown to label the apical pole of ciliated cells and to react with a 200-kD protein. This monoclonal antibody was demonstrated to be an anti-myosin from smooth muscle or from nonmuscular cells using the following criteria: On Western blots it reacted with the myosin heavy chains from gizzard and platelet extracts and from cultured cell line extracts, but did not react with striated muscle myosin heavy chains. By immunofluorescence it decorated the stress fibers of well-spread cells with a characteristic striated pattern, while it did not react with myotubes containing organized myofibrils. On native ciliated cells as well as on Triton-extracted ciliated cortices from quail oviduct, this monoclonal antibody decorated the apical pole with a stronger labeling of the periphery of the apical area. Ultrastructural localization was attempted using the immunogold technique on the same preparation. Myosin was associated with a filamentous material present between striated rootlets and the proximal extremities of the basal bodies. No labeling of the basal body itself or of axoneme was observed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.