ArticlePLoS pathogens2022
Distinct spatial arrangements of ACE2 and TMPRSS2 expression in Syrian hamster lung lobes dictates SARS-CoV-2 infection patterns.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- TMPRSS2 as a Key Player in Viral Pathogenesis: Influenza and Coronaviruses.Biomolecules · 2025Review
- Mucosal multivalent NDV-based vaccine provides cross-reactive immune responses against SARS-CoV-2 variants in animal models.Frontiers in immunology · 2025Article
- TMPRSS2-mediated SARS-CoV-2 uptake boosts innate immune activation, enhances cytopathology, and drives convergent virus evolution.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungs.Microbiology spectrum · 2024Article
- Omicron variant and pulmonary involvements: a chest imaging analysis in asymptomatic and mild COVID-19.Frontiers in public health · 2024Article
- Article
- SARS-CoV-2 Infection in Captive Hippos (Animals : an open access journal from MDPI · 2023Article
- Animal models for COVID-19 and tuberculosis.Frontiers in immunology · 2023Review
- The impact of primary immunization route on the outcome of infection with SARS-CoV-2 in a hamster model of COVID-19.Frontiers in microbiology · 2023Article
- Animal models and SARS-CoV-2-induced pulmonary and neurological injuries.Memorias do Instituto Oswaldo Cruz · 2023Review
- Protective neutralizing epitopes in SARS-CoV-2.Immunological reviews · 2022Review
- Therapeutic efficacy of monoclonal antibodies and antivirals against SARS-CoV-2 Omicron BA.1 in Syrian hamsters.Nature microbiology · 2022Article
- Article
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
SARS-CoV-2 attaches to angiotensin-converting enzyme 2 (ACE2) to gain entry into cells after which the spike protein is cleaved by the transmembrane serine protease 2 (TMPRSS2) to facilitate viral-host membrane fusion. ACE2 and TMPRSS2 expression profiles have been analyzed at the genomic, transcriptomic, and single-cell RNAseq levels. However, transcriptomic data and actual protein validation convey conflicting information regarding the distribution of the biologically relevant protein receptor in whole tissues. To describe the organ-level architecture of receptor expression, related to the ability of ACE2 and TMPRSS2 to mediate infectivity, we performed a volumetric analysis of whole Syrian hamster lung lobes. Lung tissue of infected and control animals was stained using antibodies against ACE2 and TMPRSS2, combined with SARS-CoV-2 nucleoprotein staining. This was followed by light-sheet microscopy imaging to visualize their expression and related infection patterns. The data demonstrate that infection is restricted to sites containing both ACE2 and TMPRSS2, the latter is expressed in the primary and secondary bronchi whereas ACE2 is predominantly observed in the bronchioles and alveoli. Conversely, infection completely overlaps where ACE2 and TMPRSS2 co-localize in the tertiary bronchi, bronchioles, and alveoli.
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Registered trials
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