ArticleProceedings of the National Academy of Sciences of the United States of America2022
Viable offspring derived from single unfertilized mammalian oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- First cleavage signatures define a temporal trade-off and predict embryo fitness.Scientific reports · 2026Article
- The multi-omics regulatory landscape of human early embryogenesis.Cellular and molecular life sciences : CMLS · 2026Review
- High-sensitivity in situ detection of Sox21 reveals transcript asymmetry from the zygote stage.Developmental biology · 2026Article
- Fertilization with Protamine-2 deficient sperm triggers abnormal pronucleus development and zygotic cleavage†.Biology of reproduction · 2026Article
- Epigenetic editing: from concept to clinic.Nature reviews. Drug discovery · 2026Review
- Fertile androgenetic mice generated by targeted epigenetic editing of imprinting control regions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Generation of live mice from haploid ESCs with germline-DMR deletions or switch.Cell discovery · 2025Article
- No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo.Current topics in developmental biology · 2025Review
- High estrogen during ovarian stimulation induced loss of maternal imprinted methylation that is essential for placental development via overexpression of TET2 in mouse oocytes.Cell communication and signaling : CCS · 2024Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- CRISPR/Cas9 technology: applications in oocytes and early embryos.Journal of translational medicine · 2023Review
- A genetic basis for facultative parthenogenesis in Drosophila.Current biology : CB · 2023Article
- Reproductive biology: A genetic recipe for parthenogenesis.Current biology : CB · 2023Article
- Folding makes an imprint.Genes & development · 2023Article
- Article
- Cocktails of defined chemical compounds: sufficient to induce totipotency in embryonic stem cells.Signal transduction and targeted therapy · 2022Article
- Comparative maternal protein profiling of mouse biparental and uniparental embryos.GigaScience · 2022Article
- Identification of the porcine IG-DMR and abnormal imprinting ofFrontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, a new life starts with the fusion of an oocyte and asperm cell. Parthenogenesis, a way of generating offspring solelyfrom female gametes, is limited because of problems arising fromgenomic imprinting. Here, we report live mammalian offspringderived from single unfertilized oocytes, which was achieved by tar-geted DNA methylation rewriting of seven imprinting control regions.Oocyte coinjection of catalytically inactive Cas9 (dCas9)-Dnmt3a ordCpf1-Tet1 messenger RNA (mRNA) with single-guide RNAs (sgRNAs)targeting specific regions induced de novo methylation or demethyla-tion, respectively, of the targeted region. Following parthenogeneticactivation, these edited regions showed maintenance of methylationas naturally established regions during early preimplantation develop-ment. The transfer of modified parthenogenetic embryos into fostermothers resulted in significantly extended development andfinally inthe generation of viable full-term offspring. These data demonstratethat parthenogenesis can be achieved by targeted epigenetic rewrit-ing of multiple critical imprinting control regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.