Evidence map›Paper›PMID 35254499›Full record

ArticleApplied microbiology and biotechnology2022

Core antigenic advantage domain-based ELISA to detect antibody against novel goose astrovirus in breeding geese.

Zeng Wang, Huayuan Chen, Shenyan Gao, Mingzhen Song, Zicong Shi, Zhifeng Peng, Qianyue Jin, Li Zhao, Hongxing Qiao, Chuanzhou Bian and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Zeng Wang *College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.ORCID http://orcid.org/0000-0002-3274-4296
Huayuan Chen *College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Shenyan Gao *College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Mingzhen SongCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Zicong ShiCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Zhifeng PengCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China.
Qianyue JinHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China.
Li ZhaoCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China.
Hongxing QiaoCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China.
Chuanzhou BianCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China.
Xia YangCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Xiaozhan ZhangCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, People's Republic of China. xiaozhan063@163.com.
Jun ZhaoCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China. zhaoj@henau.edu.cn.
Henan Agricultural University · CNHenan University · CNHenan Academy of Agricultural Sciences · CN

Funding

National Natural Science Foundation of China 32002259Natural Science Foundation of Henan Province 202300410198
6 · The paper itself

Abstract

Goose astrovirus (GAstV), the major causative agent of visceral and joint gout in goslings, is a novel pathogen greatly threatening waterfowl industry. Importantly, the horizontal and vertical transmissibility of GAstV posed a great challenge for disease prevention and control. Given the absence of commercial vaccine, restricting vertical transmission and protecting susceptible goslings must be a priority. Although many detection methods have been established, there is no serological method to detect GAstV-specific antibody, greatly limiting inspection and elimination of infected breeding bird. In this study, the B-cell epitopes of GAstV capsid protein were predicted, and its core antigenic advantage domain (shCAP) was expressed and purified. After authenticating the antigenicity, the recombinant shCAP protein was taken as the coating antigen, and an easily accessible indirect enzyme-linked immunosorbent assay (ELISA) was established to detect GAstV-specific antibody. The working conditions, including antigen concentration, serum dilution and incubation time, blocking buffer concentration, and color developing time, were gradually optimized by checkerboard titration. The cut-off OD

Indexed as

AvastrovirusPoultry DiseasesAnimalsAntibodiesEnzyme-Linked Immunosorbent AssayGeeseAntibodiesAntigenic advantage domainCapsid proteinGoose astrovirusIndirect ELISASerological investigation

Identifiers

PMID35254499
OpenAlexW4220921797

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.