ArticleMicrobiology spectrum2022
Quantitative High-Throughput Screening Methods Designed for Identification of Bacterial Biocontrol Strains with Antifungal Properties.
Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Biosynthetic diversity and antifungal potential of soil-derivedApplied and environmental microbiology · 2026Article
- A Complete Genome Resource and Bio-Control Activity of Soil-Isolated Pseudomonas citronellolis Strain M03 Against Onion-Pathogenic Burkholderia Species.MicrobiologyOpen · 2026Article
- Lactic Acid Bacteria for Fungal Control and Shelf-Life Extension in Fresh Pasta: Mechanistic Insights and Clean-Label Strategies.Molecules (Basel, Switzerland) · 2026Review
- Metabolite interactions mediate beneficial alliances between Bacillus and Trichoderma for effective Fusarium wilt control.The ISME journal · 2026Article
- Article
- Fishing for nutrient-competing antagonists for ginseng pathogen control via root biomass.MethodsX · 2025Article
- EndophyticMicrobiology spectrum · 2025Article
- Plipastatin is a shared good by Bacillus subtilis during combating Fusarium spp.FEMS microbiology ecology · 2025Article
- Evaluating the Role of Viable Cells, Heat-Killed Cells or Cell-Free Supernatants in Bacterial Biocontrol of Fungi: A Comparison Between Lactic Acid Bacteria andMicroorganisms · 2025Article
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
- Systematic Evaluation of Biotic and Abiotic Factors in Antifungal Microorganism Screening.Microorganisms · 2024Article
- Enhanced surface colonisation and competition during bacterial adaptation to a fungus.Nature communications · 2024Article
- AFrontiers in fungal biology · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large screens of bacterial strain collections to identify potential biocontrol agents often are time-consuming and costly and fail to provide quantitative results. In this study, we present two quantitative and high-throughput methods to assess the inhibitory capacity of bacterial biocontrol candidates against fungal phytopathogens. One method measures the inhibitory effect of bacterial culture supernatant components on the fungal growth, while the other accounts for direct interaction between growing bacteria and the fungus by cocultivating the two organisms. The antagonistic supernatant method quantifies the culture components' antifungal activity by calculating the cumulative impact of supernatant addition relative to the growth of a nontreated fungal control, while the antagonistic cocultivation method identifies the minimal bacterial cell concentration required to inhibit fungal growth by coinoculating fungal spores with bacterial culture dilution series. Thereby, both methods provide quantitative measures of biocontrol efficiency and allow prominent fungal inhibitors to be distinguished from less effective strains. The combination of the two methods sheds light on the types of inhibition mechanisms and provides the basis for further mode-of-action studies. We demonstrate the efficacy of the methods using Bacillus spp. with different levels of antifungal activities as model antagonists and quantify their inhibitory potencies against classic plant pathogens.
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