ArticleJACS Au2022
Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold.
Article in JACS Au, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Biotin- and Chloroalkane-Modified Nucleotides for Dual Display of Proteins on DNA Through Streptavidin and Halotag Binding.Angewandte Chemie (International ed. in English) · 2026Article
- Enhancing Enzyme Activity Via Noncovalent Association with DNA-Functionalized Nanoparticles.JACS Au · 2026Article
- Can intrinsic loop energetics predict G-Quadruplex topology?PLoS computational biology · 2026Article
- DNA-Enzyme Hybrid Nanostructures: Functional Materials to Modulate Enzymatic Activity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Peptide-based artificial cytoskeleton enhances colocalized cascade reactions in cell-like microreactors.Chemical science · 2026Article
- Stoichiometrically Engineered Hydrated Ionic Liquids Enabling Reinforcement of Enzyme Cascade with Improved Thermodynamic Stability.ACS sustainable chemistry & engineering · 2026Article
- Carrier-Free Enzyme Immobilization for High-Density Catalytic Architectures in Flow Biocatalysis.JACS Au · 2026Review
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- Modular programming of interaction and geometric specificity enables assembly of complex DNA origami nanostructures.Nature communications · 2025Article
- Wireframe DNA Origami Capable of Vertex-protruding Transformation.Chembiochem : a European journal of chemical biology · 2025Article
- Scalable fabrication of Chip-integrated 3D-nanostructured electronic devices via DNA-programmable assembly.Science advances · 2025Article
- Crystalline Assemblies of DNA Nanostructures and Their Functional Properties.Angewandte Chemie (International ed. in English) · 2025Review
- A DNA origami-based enzymatic cascade nanoreactor for chemodynamic cancer therapy and activation of antitumor immunity.Science advances · 2025Article
- A method for site-specifically tethering the enzyme urease to DNA origami with sustained activity.PloS one · 2025Article
- DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations.Analytical chemistry · 2024Article
- Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes.Applied biochemistry and biotechnology · 2024Review
- Article
- DNA Adjuvant Hydrogel-Optimized Enzymatic Cascade Reaction for Tumor Chemodynamic-Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells.Biosensors · 2023Review
- DNA T-shaped crossover tiles for 2D tessellation and nanoring reconfiguration.Nature communications · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works introducing enzyme organizations in 3D lattices. As the ability to define nanoscale structure has grown, it is critical to fully deconstruct the impact of enzyme organization at the single-scaffold level. Here, we present an open, three-dimensional (3D) DNA wireframe octahedron which is used to create a library of spatially arranged organizations of glucose oxidase and horseradish peroxidase. We explore the contribution of enzyme spacing, arrangement, and location on the 3D scaffold to cascade activity. The experiments provide insight into enzyme scaffold design, including the insignificance of scaffold sequence makeup on activity, an increase in activity at small enzyme spacings of <10 nm, and activity changes that arise from discontinuities in scaffold architecture. Most notably, the experiments allow us to determine that enzyme colocalization itself on the DNA scaffold dominates over any specific enzyme arrangement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.