ArticleThe Journal of biological chemistry2022
Analysis of cellular water content in T cells reveals a switch from slow metabolic water gain to rapid water influx prior to cell division.
Article in The Journal of biological chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- β-Actin Deficiency in Baraitser-Winter Syndrome Type 1 Disrupts T-Cell Function and Immune Regulation: Implications for Targeted Therapy in Actinopathies.Journal of clinical immunology · 2025Article
- WNK1-dependent water influx is required for CD4Nature communications · 2025Article
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Hunting the Cell CycleLife (Basel, Switzerland) · 2024Article
- Sufficient water intake maintains the gut microbiota and immune homeostasis and promotes pathogen elimination.iScience · 2024Article
- Dynamic changes in fungal communities and functions in different air-curing stages of cigar tobacco leaves.Frontiers in microbiology · 2024Article
- In vivo deuterium magnetic resonance imaging of xenografted tumors following systemic administration of deuterated water.Scientific reports · 2023Article
- Nuclear spin effects in biological processes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- In vivo deuterium magnetic resonance imaging of xenografted tumors following systemic administration of deuterated water.Research square · 2023Article
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Authors and funding
9 authors.
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Abstract
Cell growth is driven by the acquisition and synthesis of both dry biomass and water mass. In this study, we examine the increase of water mass in T cell during cell growth. We found that T-cell growth is characterized by an initial phase of slow increase in cellular water, followed by a second phase of rapid increase in water content. To study the origin of the water gain, we developed a novel methodology we call cold aqua trap-isotope ratio mass spectrometry, which allows analysis of the isotope composition of intracellular water. Applying cold aqua trap-isotope ratio mass spectrometry, we discovered that glycolysis-coupled metabolism of water accounts on average for 11 fl out of the 20 fl of water gained per cell during the initial slow phase. In addition, we show that at the end of the rapid phase before initiation of cell division, a water influx occurs, increasing the cellular water mass by threefold. Thus, we conclude that activated T cells switch from metabolizing water to rapidly taking up water from the extracellular medium prior to cell division. Our work provides a method to analyze cell water content as well as insights into the ways cells regulate their water mass.
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