ArticlePlant biotechnology journal2022
A rust fungus effector directly binds plant pre-mRNA splice site to reprogram alternative splicing and suppress host immunity.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Pathogen Effector-Mediated Reprogramming of Plant Alternative Splicing: From Immune Regulation to Crop Disease Resistance.Plants (Basel, Switzerland) · 2026Review
- Infection-responsive alternative splicing of GhZDS is associated with Verticillium wilt resistance in cotton.BMC plant biology · 2026Article
- The transcription factor TaWRKY58 coordinates growth and drought sensitivity in wheat by repressing TaLRR and TaBCS1.PLoS genetics · 2026Article
- Article
- Stripe rust fungus subverts wheat phase separation-mediated immunity to promote infection.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Uncovering the potential MTAs, candidate genes and microRNAs regulatory networks involved in salinity stress tolerance triggered in Iranian Aegilops tauschii.Scientific reports · 2026Article
- Dynamic Coordination of Alternative Splicing and Subgenome Expression Bias Underlies Rusty Root Symptom Response inPlants (Basel, Switzerland) · 2025Article
- A Novel CFEM Effector inInternational journal of molecular sciences · 2025Article
- Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.Molecular plant pathology · 2025Review
- A catalogue of virulence strategies mediated by phytopathogenic effectors.Fundamental research · 2025Review
- APlants (Basel, Switzerland) · 2025Article
- Differences in alternative splicing and their potential underlying factors between animals and plants.Journal of advanced research · 2024Review
- Alternative splicing regulation in plants by SP7-like effectors from symbiotic arbuscular mycorrhizal fungi.Nature communications · 2024Article
- Fine mapping a QTL for BYDV-PAV resistance in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Co-expression network analysis and identification of core genes in the interaction between wheat and Puccinia striiformis f. sp. tritici.Archives of microbiology · 2024Article
- Stripe Rust Effector Pst_9302 Inhibits Wheat Immunity to Promote Susceptibility.Plants (Basel, Switzerland) · 2023Article
- Magnaporthe oryzae effector MoSPAB1 directly activates rice Bsr-d1 expression to facilitate pathogenesis.Nature communications · 2023Article
- TaRBP1 stabilizes TaGLTP and negatively regulates stripe rust resistance in wheat.Molecular plant pathology · 2023Article
- A geraniol synthase regulates plant defense via alternative splicing in tea plants.Horticulture research · 2023Article
- Uncovering genomic and transcriptional variations facilitates utilization of wild resources in cotton disease resistance improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing (AS) is a crucial post-transcriptional regulatory mechanism in plant resistance. However, whether and how plant pathogens target splicing in their host remains mostly unknown. For example, although infection by Puccinia striiformis f. sp. tritici (Pst), a pathogenic fungus that severely affects the yield of wheat worldwide, has been shown to significantly influence the levels of alternatively spliced transcripts in the host, the mechanisms that govern this process, and its functional consequence have not been examined. Here, we identified Pst_A23 as a new Pst arginine-rich effector that localizes to host nuclear speckles, nuclear regions enriched in splicing factors. We demonstrated that transient expression of Pst_A23 suppresses plant basal defence dependent on the Pst_A23 nuclear speckle localization and that this protein plays an important role in virulence, stable silencing of which improves wheat stripe rust resistance. Remarkably, RNA-Seq data revealed that AS patterns of 588 wheat genes are altered in Pst_A23-overexpressing lines compared to control plants. To further examine the direct relationship between Pst_A23 and AS, we confirmed direct binding between two RNA motifs predicted from these altered splicing sites and Pst_A23 in vitro. The two RNA motifs we chose occur in the cis-element of TaXa21-H and TaWRKY53, and we validated that Pst_A23 overexpression results in decreased functional transcripts of TaXa21-H and TaWRKY53 while silencing of TaXa21-H and TaWRKY53 impairs wheat resistance to Pst. Overall, this represents formal evidence that plant pathogens produce 'splicing' effectors, which regulate host pre-mRNA splicing by direct engagement of the splicing sites, thereby interfering with host immunity.
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