Evidence map›Paper›PMID 35247199›Full record

ArticleTissue engineering and regenerative medicine2022

Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Anti-oxidative Ability and Differentiation Potential.

Szu-Hsien Wu, Jin-Huei Yu, Yu-Ting Liao, Kuo-Hao Liu, En-Rung Chiang, Ming-Chau Chang, Jung-Pan Wang

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Szu-Hsien Wu *Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, 112, Taiwan.
Jin-Huei Yu *Department of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33004, Taiwan.
Yu-Ting LiaoDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, 112, Taiwan.
Kuo-Hao LiuDepartment of Orthopaedics, National Yang Ming Chiao Tung University Hospital, Yilan, 260, Taiwan.
En-Rung ChiangDepartment of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Ming-Chau ChangDepartment of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Jung-Pan WangDepartment of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan. jpwang@vghtpe.gov.tw.ORCID http://orcid.org/0000-0002-8427-9963
National Yang Ming Chiao Tung University · TWTaipei Veterans General Hospital · TWTao Yuan General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInfant adipose-derived mesenchymal stem cells (ADSCs) collected from excised polydactyly fat tissue, which was surgical waste, could be cultured and expanded in vitro in this study. In addition, the collecting process would not cause pain in the host. In this study, the proliferation, reduction of senescence, anti-oxidative ability, and differentiation potential in the infant ADSCs were compared with those in the adult ADSCs harvested from thigh liposuction to determine the availability of infant ADSCs.

methodsProliferation was determined by detecting the fold changes in cell numbers and doubling time periods. Senescence was analyzed by investigating the age-related gene expression levels and the replicative stress. The superoxide dismutase (SOD) gene expression, adipogenic, neurogenic, osteogenic, and tenogenic differentiation were compared by RT-qPCR. The chondrogenic differentiation efficiency was also determined using RT-qPCR and immunohistochemical staining.

resultsThe proliferation, SOD (SOD1, SOD2 and SOD3) gene expression, the stemness-related gene (c-MYC) and telomerase reverse transcriptase of the infant ADSCs at early passages were enhanced compared with those of the adults'. Cellular senescence related genes, including p16, p21 and p53, and replicative stress were reduced in the infant ADSCs. The adipogenic genes (PPARγ and LPL) and neurogenic genes (MAP2 and NEFH) of the infant ADSC differentiated cells were significantly higher than those of the adults' while the expression of the osteogenic genes (OCN and RUNX) and tenogenic genes (TNC and COL3A1) of both demonstrated opposite results. The chondrogenic markers (SOX9, COL2 and COL10) were enhanced in the infant ADSC differentiated chondrogenic pellets, and the expression levels of SODs were decreased during the differentiation process.

conclusionCultured infant ADSCs demonstrate less cellular senescence and replicative stress, higher proliferation rates, better antioxidant defense activity, and higher potential of chondrogenic, adipogenic and neurogenic differentiation.

Indexed as

Mesenchymal Stem CellsAdipose TissueAdultCell DifferentiationCell ProliferationHumansSuperoxide DismutaseSuperoxide DismutaseAdipose-derived mesenchymal stem cells (ADSCs)Chondrogenic differentiationInfant ADSCs

Identifiers

PMID35247199
PMCPMC9130449
OpenAlexW4220916556

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.