ArticleNature communications2022
In vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterization.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 52 citations in OpenAlex.
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- Long-term feeder cell-free cat intestinal organoid cultures to study Toxoplasma gondii's sexual development.Communications biology · 2026Article
- Bradyzoite subtypes rule the crossroads of Toxoplasma development.Nature communications · 2026Article
- Dense granule proteins: key mediators ofFrontiers in medicine · 2026Review
- Toxoplasma gondii infection inhibits myotube formation in a 3D model of chicken muscle cell culture.BMC microbiology · 2025Article
- Mapping amBio · 2025Article
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- GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies.Nature communications · 2025Article
- High-Throughput Repurposing Screen Reveals Compounds with Activity againstACS infectious diseases · 2025Article
- Modeling Toxoplasma gondii-gut early interactions using a human microphysiological system.PLoS neglected tropical diseases · 2025Article
- Mitochondria of a human skeletal muscle cell line associate with the parasitophorous vacuoles of three archetypal lineages ofFEMS microbes · 2025Article
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- High Throughput Repurposing Screen Reveals Compounds with Activity AgainstbioRxiv : the preprint server for biology · 2024Article
- Functional Characterization of Six Eukaryotic Translation Initiation Factors ofInternational journal of molecular sciences · 2024Article
- A myzozoan-specific protein is an essential membrane-anchoring component of the succinate dehydrogenase complex inOpen biology · 2024Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. However, current in vitro models do not allow long-term culture of these cysts to maturity. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts that are orally infectious to mice and tolerate exposure to a range of antibiotics and temperature stresses. Metabolomic characterization of purified cysts reveals global changes that comprise increased levels of amino acids and decreased abundance of nucleobase- and tricarboxylic acid cycle-associated metabolites. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. Direct access to persistent stages of T. gondii under defined cell culture conditions will be essential for the dissection of functionally important host-parasite interactions and drug evasion mechanisms. It will also facilitate the identification of new strategies for therapeutic intervention.
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