Evidence map›Paper›PMID 35245720›Full record

ArticlePlacenta2022

Assessing hypoxic damage to placental trophoblasts by measuring membrane viscosity of extracellular vesicles.

Changjin Huang, Hui Li, Juliana S Powell, Yingshi Ouyang, Stacy G Wendell, Subra Suresh, K Jimmy Hsia, Yoel Sadovsky, David Quinn

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Placenta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Changjin HuangSchool of Mechanical and Aerospace Engineering, Nanyang Technological University, Republic of Singapore. Electronic address: cjhuang@ntu.edu.sg.
Hui LiMagee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA; Reproductive Department of Xiangya Hospital, Central South University, Changsha, Hunan, China; The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Juliana S PowellMagee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
Yingshi OuyangMagee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
Stacy G WendellDepartment of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Subra SureshNanyang Technological University, Republic of Singapore.
K Jimmy HsiaSchool of Mechanical and Aerospace Engineering, Nanyang Technological University, Republic of Singapore; School of Chemical and Biomedical Engineering, Nanyang Technological University, Republic of Singapore. Electronic address: kjhsia@ntu.edu.sg.
Yoel SadovskyMagee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, USA; Department of Microbiology and Molecular Genetics, University of Pittsburgh, PA, USA. Electronic address: ysadovsky@mwri.magee.edu.
David QuinnDepartment of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA. Electronic address: djquinn@andrew.cmu.edu.
University of Pittsburgh · USNanyang Technological University · SGCarnegie Mellon University · USCentral South University · CN

Funding

Extracellular vesicles and their ncRNA cargo as markers of trophoblast injuryR01HD086325 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI SADOVSKY, YOEL · 2015 to 2019
$3.6M
Exosome Based Placental Maternal CommunicationR37HD086916 · NICHD · STANFORD UNIVERSITY · PI Yoel Sadovsky · 2017 to 2026
$3.5M
Bringing Untargeted Metabolomics to PittS10OD023402 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GELHAUS, STACY LYNN · 2018 to 2018
$594k
NICHD NIH HHS R01 HD086325NICHD NIH HHS R37 HD086916NIH HHS S10 OD023402
6 · The paper itself

Abstract

introductionAs highly sophisticated intercellular communication vehicles in biological systems, extracellular vesicles (EVs) have been investigated as both promising liquid biopsy-based disease biomarkers and drug delivery carriers. Despite tremendous progress in understanding their biological and physiological functions, mechanical characterization of these nanoscale entities remains challenging due to the limited availability of proper techniques. Especially, whether damage to parental cells can be reflected by the mechanical properties of their EVs remains unknown.

methodsIn this study, we characterized membrane viscosities of different types of EVs collected from primary human trophoblasts (PHTs), including apoptotic bodies, microvesicles and small extracellular vesicles, using fluorescence lifetime imaging microscopy (FLIM). The biochemical origin of EV membrane viscosity was examined by analyzing their phospholipid composition, using mass spectrometry.

resultsWe found that different EV types derived from the same cell type exhibit different membrane viscosities. The measured membrane viscosity values are well supported by the lipidomic analysis of the phospholipid compositions. We further demonstrate that the membrane viscosity of microvesicles can faithfully reveal hypoxic injury of the human trophoblasts. More specifically, the membrane of PHT microvesicles released under hypoxic condition is less viscous than its counterpart under standard culture condition, which is supported by the reduction in the phosphatidylethanolamine-to-phosphatidylcholine ratio in PHT microvesicles. DISCUSSION: Our study suggests that biophysical properties of released trophoblastic microvesicles can reflect cell health. Characterizing EV's membrane viscosity may pave the way for the development of new EV-based clinical applications.

Indexed as

Extracellular VesiclesTrophoblastsDrug CarriersFemaleHumansPhospholipidsPlacentaPregnancyViscosityDrug CarriersPhospholipidsExtracellular vesicleFluorescence lifetimeHypoxiaMembrane viscosityPlacental trophoblast

Identifiers

PMID35245720
PMCPMC9010367
OpenAlexW4213425855

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.