Evidence map›Paper›PMID 35245632›Full record

ArticleJournal of ethnopharmacology2022

Probing PXR activation and modulation of CYP3A4 by Tinospora crispa and Tinospora sinensis.

Abidah Parveen, Manal Alhusban, Omer I Fantoukh, Zulfiqar Ali, Amar G Chittiboyina, Ikhlas A Khan, Shabana I Khan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ethnopharmacology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

Abidah ParveenNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, United States; Department of Pharmaceutical Sciences, Abbottabad University of Science & Technology, Havelian, KPK, Pakistan. Electronic address: abidahp@gmail.com.
Manal AlhusbanNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, United States; Faculty of Pharmacy, Philadelphia University, Amman, Jordan. Electronic address: Mmkhusban@gmail.com.
Omer I FantoukhDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Electronic address: ofantoukh@ksu.edu.sa.
Zulfiqar AliNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States. Electronic address: zulfiqar@olemiss.edu.
Amar G ChittiboyinaNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States. Electronic address: amar@olemiss.edu.
Ikhlas A KhanNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, United States. Electronic address: ikhan@olemiss.edu.
Shabana I KhanNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, United States. Electronic address: skhan@olemiss.edu.
University of Mississippi · USAbbottabad University of Science and Technology · PKKing Saud University · SAPhiladelphia University · JO

Funding

Science based authentication, validation & safety of botanical ingredientsU01FD004246 · FDA · UNIVERSITY OF MISSISSIPPI · PI Amar G. Chittiboyina, Ikhlas Ahmad Khan · 2011 to 2026
$38.2M
FDA HHS U01 FD004246
6 · The paper itself

Abstract

ethnopharmacological relevanceThe two Tinospora species, T. crispa and T. sinensis, native to Southeast Asia, are integral components of various traditional preparations with structure-function claims to treat various disorders, including diabetes and inflammation. AIM OF THE STUDY: To assure the safety of the botanicals finished products, herb-drug interaction potential of T. crispa and T. sinensis was investigated by testing their extracts and compounds for in vitro activation of the pregnane X-receptor (PXR) and the modulation of CYP3A4 isozyme, selectively. MATERIALS AND

methodsA total of sixteen fully characterized phytochemicals from T. crispa and T. sinensis were evaluated for PXR activation by luciferase reporter gene assay. CYP3A4 inhibition studies were carried out for eleven compounds. In addition, docking studies were performed to elucidate the possible binding modes to the PXR by the compounds using computational methods.

resultsSignificant activation of PXR (2-fold) was observed for both extracts and non-polar fractions of T. crispa. Among the pure compounds, columbin showed highest activation of PXR (3-fold), which was comparable with the positive control, rifampicin. Vital interactions were predicted with docking simulation of PXR-columbin complex with critical amino acid residues (Trp-299) that are known for the activation of PXR. The methanolic extracts of T. crispa and T. sinensis also showed considerable CYP3A4 inhibition.

conclusionT. crispa and T. sinensis, both demonstrated the potential to mediate herb-drug interaction through PXR activation and inhibition of CYP3A4 isozyme. Moreover, the elucidation of the potential to induce herb-drug interaction, by the phytochemicals of these Tinospora plants, thereby supports the need for further investigation to establish the clinical relevancy of these constituents for possible adverse interactions with pharmaceutical drugs.

Indexed as

Diabetes MellitusReceptors, SteroidTinosporaCytochrome P-450 CYP3AHerb-Drug InteractionsHumansPlant ExtractsCYP3A4 protein, humanCytochrome P-450 CYP3APlant ExtractsReceptors, SteroidColumbinFuranoditerpenoidsHerb-drug interactionTinospora crispaTinospora sinensis

Identifiers

PMID35245632
PMCPMC11094663
OpenAlexW4214747170

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.