ArticlePloS one2022
Functional disruption of transferrin expression alters reproductive physiology in Anopheles culicifacies.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Knockdown of the adipokinetic hormone receptor inhibits the reproduction of female Culex pipiens pallens (Diptera: Culicidae) by downregulating L-homoserine and serotonin levels.Insect science · 2026Article
- Iron Homeostasis and Reproduction: Unveiling the Microbiome-Gut-Brain Axis Connection in the MosquitoCells · 2026Article
- Immune-reproductive cross talk in mosquitoes: molecular pathways and energy homeostasis.Parasites & vectors · 2026Review
- Mechanisms and targets of harnessingJournal of biomedical research · 2026Article
- Mating triggers a physiological switch to reproduction in field crickets to mitigate the flight-oogenesis tradeoff.Current research in insect science · 2026Article
- Thermal variation influences the transcriptome of the major malaria vector Anopheles stephensi.Communications biology · 2025Article
- Sensory appendage protein triggers alarm to pyrethroid in Indian malarial vector Anopheles culicifacies.PloS one · 2025Article
- Mosquito gene targeted RNAi studies for vector control.Functional & integrative genomics · 2023Review
- Metal ions in insect reproduction: a crosstalk between reproductive physiology and immunity.Current opinion in insect science · 2022Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIron metabolism is crucial to maintain optimal physiological homeostasis of every organism and any alteration of the iron concentration (i.e. deficit or excess) can have adverse consequences. Transferrins are glycoproteins that play important role in iron transportation and have been widely characterized in vertebrates and insects, but poorly studied in blood-feeding mosquitoes.
resultsWe characterized a 2102 bp long transcript AcTrf1a with complete CDS of 1872bp, and 226bp UTR region, encoding putative transferrin homolog protein from mosquito An. culicifacies. A detailed in silico analysis predicts AcTrf1a encodes 624 amino acid (aa) long polypeptide that carries transferrin domain. AcTrf1a also showed a putative N-linked glycosylation site, a characteristic feature of most of the mammalian transferrins and certain non-blood feeding insects. Structure modelling prediction confirms the presence of an iron-binding site at the N-terminal lobe of the transferrin. Our spatial and temporal expression analysis under altered pathophysiological conditions showed that AcTrf1a is abundantly expressed in the fat-body, ovary, and its response is significantly altered (enhanced) after blood meal uptake, and exogenous bacterial challenge. Additionally, non-heme iron supplementation of FeCl3 at 1 mM concentration not only augmented the AcTrf1a transcript expression in fat-body but also enhanced the reproductive fecundity of gravid adult female mosquitoes. RNAi-mediated knockdown of AcTrf1a causes a significant reduction in fecundity, confirming the important role of transferrin in oocyte maturation.
conclusionAll together our results advocate that detailed characterization of newly identified AcTrf1a transcript may help to select it as a unique target to impair the mosquito reproductive outcome.
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