ArticleScience immunology2022
Cysteinyl leukotrienes and acetylcholine are biliary tuft cell cotransmitters.
Article in Science immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 49 citations in OpenAlex.
- Vagal sensory neurons profusely innervate the mouse gall bladder.Autonomic neuroscience : basic & clinical · 2026Article
- Gastrointestinal tuft cells: Recent insights and next questions.Science immunology · 2026Review
- Article
- Tuft cells shape airway remodeling by eliciting OXGR1- and SOX9-dependent stem cell programs.Nature communications · 2026Article
- Histological, ultrastructural, and single-cell profiling reveal immune-mediated remodeling in gallbladder inflammation.Cell and tissue research · 2026Article
- Protocadherin 20 Is a POU Class 2 Homeobox 3 Target Gene Required for Proper Tuft Cell Microvillus Organization.Cellular and molecular gastroenterology and hepatology · 2026Article
- Tuft cell-mediated pathogen sensing and inflammation resolution.Biochemical Society transactions · 2025Review
- Using gnotobiotic mice to decipher effects of gut microbiome repair in undernourished children on tuft and goblet cell function.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unraveling cysteinyl leukotrienes and their receptors in inflammation through the brain-gut-lung axis.Virulence · 2025Review
- Physiological functions and pharmacological targeting of transient receptor potential channels.Pharmacological reviews · 2025Review
- The Central Cholinergic Synapse: A Primer.International journal of molecular sciences · 2025Review
- Using gnotobiotic mice to decipher effects of gut microbiome repair in undernourished children on tuft and goblet cell function.bioRxiv : the preprint server for biology · 2025Article
- Differential expression of villin and advillin by neuroendocrine and tuft cells in the murine lower airways.Cell and tissue research · 2025Article
- Cellular heterogeneity and patterning strategies as revealed by upper respiratory epithelium single cell atlas.iScience · 2025Article
- Tuft cells in the intestine, immunity and beyond.Nature reviews. Gastroenterology & hepatology · 2024Review
- Article
- Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors.Scientific reports · 2024Article
- Weep and sweep and the broom: tuft cell acetylcholine limits the worm.Trends in parasitology · 2024Article
- Tuft cell-derived acetylcholine promotes epithelial chloride secretion and intestinal helminth clearance.Immunity · 2024Article
- G protein subunit GeLife · 2024Article
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Authors and funding
26 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gallbladder stores bile between meals and empties into the duodenum upon demand and is thereby exposed to the intestinal microbiome. This exposure raises the need for antimicrobial factors, among them, mucins produced by cholangiocytes, the dominant epithelial cell type in the gallbladder. The role of the much less frequent biliary tuft cells is still unknown. We here show that propionate, a major metabolite of intestinal bacteria, activates tuft cells via the short-chain free fatty acid receptor 2 and downstream signaling involving the cation channel transient receptor potential cation channel subfamily M member 5. This results in corelease of acetylcholine and cysteinyl leukotrienes from tuft cells and evokes synergistic paracrine effects upon the epithelium and the gallbladder smooth muscle, respectively. Acetylcholine triggers mucin release from cholangiocytes, an epithelial defense mechanism, through the muscarinic acetylcholine receptor M3. Cysteinyl leukotrienes cause gallbladder contraction through their cognate receptor CysLTR1, prompting emptying and closing. Our results establish gallbladder tuft cells as sensors of the microbial metabolite propionate, initiating dichotomous innate defense mechanisms through simultaneous release of acetylcholine and cysteinyl leukotrienes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.