Evidence map›Paper›PMID 35243378›Full record

ArticleSTAR protocols2022

High-resolution intravital imaging of the murine hypothalamus using GRIN lenses and confocal microscopy.

Liliia I Butiaeva, Maia V Kokoeva

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Liliia I ButiaevaDivision of Endocrinology, Department of Medicine, McGill University Health Center Research Institute, McGill University, Montreal, QC H4A 3J1, Canada.
Maia V KokoevaDivision of Endocrinology, Department of Medicine, McGill University Health Center Research Institute, McGill University, Montreal, QC H4A 3J1, Canada.

Funding

CIHR PJT-148790
6 · The paper itself

Abstract

Intravital studies of cellular morphology in structures such as the hypothalamus are challenging because of their location at the bottom of the brain. Here, we describe an intravital imaging protocol based on gradient refractive index (GRIN) lenses in conjunction with confocal microscopy to inspect fluorescent cells at high resolution in deep-brain areas. The approach relies on implanted guide-tubes for the interchangeable use of GRIN lenses, thereby allowing imaging at different magnifications and increasing the effective field of view. For complete details on the use and execution of this profile, please refer to Butiaeva et al. (2021).

Indexed as

LensesRefractometryAnimalsHypothalamusIntravital MicroscopyMiceMicroscopy, ConfocalMetabolismMicroscopyModel OrganismsNeuroscience

Identifiers

PMID35243378
PMCPMC8873921

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.