ArticleGenome biology2022
Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.
Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Roles of meiotic and mitotic cohesin in stem cell gene regulation and genome stability.Molecular therapy. Nucleic acids · 2026Review
- Diverse Roles of Cohesin in Chromosome Dynamics and Stem Cells.Biotech (Basel (Switzerland)) · 2026Review
- Amphiregulin drives EGFR-dependent genome stability in colorectal cancer and represents a targetable vulnerability.Oncogene · 2026Article
- Orchestrating homolog segregation in meiosis I: molecular logic and regulatory networks with emphasis on male metaphase I.Cell communication and signaling : CCS · 2026Review
- Assessing SMC Complex Function in Replication Fork Progression with DNA Fiber Assays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- STAG2-truncating variants reveal a mosaic STAG2 inactivation pattern and compensatory mechanisms involving cohesin complex remodeling.iScience · 2025Article
- Identification of genetic variants in patients with primary and secondary amenorrhea.Saudi medical journal · 2025Article
- Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.Scientific reports · 2025Article
- Follicular metabolic dysfunction, oocyte aneuploidy and ovarian aging: a review.Journal of ovarian research · 2025Review
- Emerging roles of cohesin-STAG2 in cancer.Oncogene · 2025Review
- The Functions and Mechanisms of the Cohesin Complex in Regulating the Fate Determinations of Stem Cells.Research (Washington, D.C.) · 2025Review
- Synergistic combination of RAD51-SCR7 improves CRISPR-Cas9 genome editing efficiency by preventing R-loop accumulation.Molecular therapy. Nucleic acids · 2024Article
- RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2024Article
- Genetic insights into the complexity of premature ovarian insufficiency.Reproductive biology and endocrinology : RB&E · 2024Review
- Mechanism of chromosomal mosaicism in preimplantation embryos and its effect on embryo development.Journal of assisted reproduction and genetics · 2024Review
- Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency.BMB reports · 2023Article
- α-Kleisin subunit of cohesin preserves the genome integrity of embryonic stem cells.BMB reports · 2023Article
- Cohesin controls X chromosome structure remodeling and X-reactivation during mouse iPSC-reprogramming.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells.Molecules and cells · 2022Article
- From the Understanding of Maternal Molecules and Mechanisms to Predicting Embryonic Development.Reproductive medicine and biologyReview
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCohesin is a chromosome-associated SMC-kleisin complex that mediates sister chromatid cohesion, recombination, and most chromosomal processes during mitosis and meiosis. However, it remains unclear whether meiosis-specific cohesin complexes are functionally active in mitotic chromosomes.
resultsThrough high-resolution 3D-structured illumination microscopy (3D-SIM) and functional analyses, we report multiple biological processes associated with the meiosis-specific cohesin components, α-kleisin REC8 and STAG3, and the distinct loss of function of meiotic cohesin during the cell cycle of embryonic stem cells (ESCs). First, we show that STAG3 is required for the efficient localization of REC8 to the nucleus by interacting with REC8. REC8-STAG3-containing cohesin regulates topological properties of chromosomes and maintains sister chromatid cohesion. Second, REC8-cohesin has additional sister chromatid cohesion roles in concert with mitotic RAD21-cohesin on ESC chromosomes. SIM imaging of REC8 and RAD21 co-staining revealed that the two types of α-kleisin subunits exhibited distinct loading patterns along ESC chromosomes. Third, knockdown of REC8 or RAD21-cohesin not only leads to higher rates of premature sister chromatid separation and delayed replication fork progression, which can cause proliferation and developmental defects, but also enhances chromosome compaction by hyperloading of retinoblastoma protein-condensin complexes from the prophase onward.
conclusionsOur findings indicate that the delicate balance between mitotic and meiotic cohesins may regulate ESC-specific chromosomal organization and the mitotic program.
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