Evidence map›Paper›PMID 35241080›Full record

ArticleMalaria journal2022

Genetic polymorphisms in genes associated with drug resistance in Plasmodium vivax parasites from northeastern Myanmar.

Fang Huang, Shigang Li, Peng Tian, Lahpai Ja Seng Pu, Yanwen Cui, Hui Liu, Lianzhi Yang, Dahidam Yaw Bi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Genetic Diversity of Potential Drug Resistance Markers inPathogens (Basel, Switzerland) · 2025
    Article
  3. Investigation of Mutations in theMicroorganisms · 2024
    Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. PvMSP-3α and PvMSP-3β genotyping reveals higher genetic diversity in Plasmodium vivax parasites from migrant workers than residents at the China-Myanmar border.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Fang HuangNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China. huangfang@nipd.chinacdc.cn.ORCID http://orcid.org/0000-0003-1105-0874
Shigang LiYingjiang County Center for Disease Control and Prevention, Yingjiang, Yunnan, China.
Peng TianYunnan Institute of Parasitic Diseases, Pu'er, Yunnan, China.
Lahpai Ja Seng PuLaiza Central Civilian Hospital, Laiza, Myanmar.
Yanwen CuiNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China.
Hui LiuYunnan Institute of Parasitic Diseases, Pu'er, Yunnan, China.
Lianzhi YangNabang Township Hospital, Yingjiang, Yunnan, China.
Dahidam Yaw BiLaiza Central Ministry of Public Health, Laiza, Myanmar.
National Institute for Parasitic Diseases · CNYunnan Institute of Parasitic Diseases · CNMinistry of Health · MMYunnan Center for Disease Control And Prevention · CN

Funding

National Important Scientific & Technological Project 2018ZX10101002-002Natural Science Foundation of Shanghai 18ZR1443400the Fifth Round of Three-Year Public Health Action Plan of Shanghai GWV-10.1-XK13
6 · The paper itself

Abstract

backgroundAnti-malarial drug resistance is still a major threat to malaria elimination in the Great Mekong Sub-region. Plasmodium vivax parasites resistant to anti-malarial drugs are now found in Myanmar. Molecular surveillance on drug resistance genes in P. vivax parasites from northeastern Myanmar was aimed at estimating the underlying drug resistance in this region.

methodsBlood samples from patients with vivax malaria were collected from Laiza city in northeastern Myanmar in 2020. Drug resistance genes including Pvcrt-o, Pvmdr1, Pvdhfr and Pvdhps were amplified and sequenced. Genetic polymorphisms and haplotypes were analysed to evaluate the prevalence of mutant alleles associated with drug resistance.

resultsA total of 149 blood samples from P. vivax patients were collected. The prevalence of Pvmdr1 mutations at codons 958 and 1076 was 100.0% and 52.0%, respectively, whereas no single nucleotide polymorphism was present at codon 976. The proportions of single and double mutant types were 48.0% and 52.0%, respectively. A K10 "AAG" insertion in the Pvcrt-o gene was not detected. Mutations in Pvdhfr at codons 57, 58, 61, 99 and 117 were detected in 29.9%, 54.3%, 27.6%, 44.9% and 55.1% of the samples, respectively. Wild type was predominant (46.3%), followed by quadruple and double mutant haplotypes. Of three types of tandem repeat variations of Pvdhfr, Type B, with three copies of GGDN repeats, was the most common. Pvdhps mutations were only detected at codons 383 and 553 and the wild type Pvdhps was dominant (78.0%). Eleven haplotypes were identified when combining the mutations of Pvdhfr and Pvdhps, among which the predominant one was the wild type (33.9%), followed by double mutant alleles S58R/S117N /WT (24.6%).

conclusionsThis study demonstrated resistant P. vivax phenotypes exists in northeastern Myanmar. Continued surveillance of drug resistance markers is needed to update treatment guidelines in this region.

Indexed as

AntimalarialsMalaria, VivaxDrug ResistanceHumansMutationMyanmarPlasmodium vivaxPolymorphism, Single NucleotideProtozoan ProteinsAntimalarialsProtozoan ProteinsDrug resistanceGenetic polymorphismsNortheastern MyanmarPlasmodium vivax

Identifiers

PMID35241080
PMCPMC8892751
OpenAlexW4214907960

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.