Evidence map›Paper›PMID 35240021›Full record

ArticleAnimal bioscience2022

Development of novel microsatellite markers to analyze the genetic structure of dog populations in Taiwan.

Fang-Yu Lai, Yu-Chen Lin, Shih-Torng Ding, Chi-Sheng Chang, Wi-Lin Chao, Pei-Hwa Wang

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Article in Animal bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Fang-Yu LaiKey Laboratory of Animal Genetics, Breeding and Bioresources, Department of Animal Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10672, Taiwan.
Yu-Chen LinKey Laboratory of Animal Genetics, Breeding and Bioresources, Department of Animal Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10672, Taiwan.
Shih-Torng DingKey Laboratory of Animal Genetics, Breeding and Bioresources, Department of Animal Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10672, Taiwan.
Chi-Sheng ChangDepartment of Animal Science, Chinese Culture University, Taipei 11114, Taiwan.
Wi-Lin ChaoDepartment of Animal Industry, Council of Agriculture, Taipei 100212., Taiwan.
Pei-Hwa WangKey Laboratory of Animal Genetics, Breeding and Bioresources, Department of Animal Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei 10672, Taiwan.

Funding

Council of Agriculture, Executive Yuan, Taiwan 2012 AS-2.1.9-AD-U1Council of Agriculture, Executive Yuan, Taiwan 2013 AS-2.1.9-AD-U1
6 · The paper itself

Abstract

objectiveAlongside the rise of animal-protection awareness in Taiwan, the public has been paying more attention to dog genetic deficiencies due to inbreeding in the pet market. The goal of this study was to isolate novel microsatellite markers for monitoring the genetic structure of domestic dog populations in Taiwan.

methodsA total of 113 DNA samples from three dog breeds-beagles (BEs), bichons (BIs), and schnauzers (SCs)-were used in subsequent polymorphic tests applying the 14 novel microsatellite markers that were isolated in this study.

resultsThe results showed that the high level of genetic diversity observed in these novel microsatellite markers provided strong discriminatory power. The estimated probability of identity (P(ID)) and the probability of identity among sibs (P(ID)sib) for the 14 novel microsatellite markers were 1.7×10-12 and 1.6×10-5, respectively. Furthermore, the power of exclusion for the 14 novel microsatellite markers was 99.98%. The neighbor-joining trees constructed among the three breeds indicated that the 14 sets of novel microsatellite markers were sufficient to correctly cluster the BEs, BIs, and SCs. The principal coordinate analysis plot showed that the dogs could be accurately separated by these 14 loci based on different breeds; moreover, the Beagles from different sources were also distinguished. The first, the second, and the third principal coordinates could be used to explain 44.15%, 26.35%, and 19.97% of the genetic variation.

conclusionThe results of this study could enable powerful monitoring of the genetic structure of domestic dog populations in Taiwan.

Indexed as

DogNovel Microsatellite MarkerPopulation Genetic Analysis

Identifiers

PMID35240021
PMCPMC9449399

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