ArticleAnimal bioscience2022
Effect of lactoferrin on ram sperm motility after cryopreservation.
Article in Animal bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Effects of Kaempferol Supplementation on the Cryopreservation Quality of Semen from Yuansheng Aite Dairy Rams.Antioxidants (Basel, Switzerland) · 2026Article
- Article
- Proteome of seminal plasma and sperm associated with sperm survival following cryopreservation in the Red Wolf (Canis rufus).Scientific reports · 2025Article
- The Equilibrium of Bacterial Microecosystem: Probiotics, Pathogenic Bacteria, and Natural Antimicrobial Substances in Semen.Microorganisms · 2024Review
- Molecular Biomarkers of Canine Reproductive Functions.Current issues in molecular biology · 2024Review
- Analysis of sperm separation protocols for isolating cryopreserved human spermatozoa.Reproduction & fertility · 2023Article
- Lactoferrin: A glycoprotein that plays an active role in human health.Frontiers in nutrition · 2022Review
- Assessment of Seminal Lactoferrin Levels in Oligoasthenoterato-zoospermic Men with Varicocele.Journal of reproduction & infertilityArticle
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Authors and funding
5 authors.
Funding
Abstract
objectiveThe objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro.
methodsSperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 μg/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS).
resultsCryopreservation significantly altered the abundance of 40 proteins; the abundance of 16 proteins was increased, while that of 24 proteins was decreased. Next, LTF was added to Tris extender applied to ram sperm. The results showed that sperm motility and plasma membrane integrity were significantly improved (p<0.05) by supplementation with 10 μg/mL LTF compared to those in the control group. There was no significant difference in mitochondrial activity between the 0 μg/mL group and other groups (p>0.05). Supplementation of the cryoprotective extender with 10 μg/mL LTF led to decreased ROS levels compared with those in the control and other groups (p<0.05).
conclusionThe LTF is an important protein during cryopreservation, and the addition of 10 μg/mL LTF to a cryoprotective extender can significantly improve the function of frozen ram sperm.
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