ArticleAnimal bioscience2022
Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites.
Article in Animal bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 12 citations in OpenAlex.
- Composite probiotics improve growth performance and enhance immune and antioxidant capacity in weaned yaks by modulating rumen microbiota and their metabolites.BMC microbiology · 2026Article
- Effect of sorghum-based diets on the abundance and diversity of ruminal methanogenic archaea in Mashona goats.Frontiers in microbiology · 2026Article
- Biochanin A improves nitrogen utilization efficiency by regulating ruminal microbial community in dairy goats.Microbiome · 2025Article
- Reducing crude protein content in the diet of lactating dairy cows improved nitrogen-use-efficiency and reduced N excretion in urine, whilst having no obvious effects on the rumen microbiome.Journal of animal science and biotechnology · 2025Article
- Comparative Analysis of Fecal Microbiota and Metabolomic Profiles in Male and Female Leizhou Goats Offered a 10% Crude Protein Diet Among Four Energy Levels.Animals : an open access journal from MDPI · 2025Article
- Multi-Omics Revealed the Effects of Different Feeding Systems on Rumen Microorganisms, Cellulose Degradation, and Metabolites in Mongolian Cattle.Animals : an open access journal from MDPI · 2025Article
- Effects of Dietary Protein Levels on Production Performance, Meat Quality Traits, and Gut Microbiome of Fatting Dezhou Donkeys.Microorganisms · 2025Article
- The Jarman-Bell principle revisited: Gut microbiota facilitate body size-dependent nutritional strategies in herbivores.Current research in microbial sciences · 2025Article
- Integrating 16S rRNA Sequencing and LC-MS-Based Metabolomics to Evaluate the Effects of Dietary Crude Protein on Ruminal Morphology, Fermentation Parameter and Digestive Enzyme Activity in Tibetan Sheep.Animals : an open access journal from MDPI · 2024Article
- Effects of yeast culture supplementation on milk yield, rumen fermentation, metabolism, and bacterial composition in dairy goats.Frontiers in veterinary science · 2024Article
- Estimating Microbial Protein Synthesis in the Rumen-Can 'Omics' Methods Provide New Insights into a Long-Standing Question?Veterinary sciences · 2023Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveThe objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats.
methodsIn an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome.
resultsThe concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments.
conclusionDecreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.
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