ArticleMolecular plant pathology2022
Two independent approaches converge to the cloning of a new Leptosphaeria maculans avirulence effector gene, AvrLmS-Lep2.
Article in Molecular plant pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- Conserved 'Late' Effector Genes From Leptosphaeria maculans Inducing Gene-For-Gene Quantitative Resistance in Brassica napus Semi-Winter Genotypes.Molecular plant pathology · 2026Article
- To be or not to be a nonhost species: A case study of the Leptosphaeria maculans and Brassica carinata interaction.Environmental microbiology reports · 2024Article
- Breeding and management of major resistance genes to stem canker/blackleg in Brassica crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- The neighbouring genes AvrLm10A and AvrLm10B are part of a large multigene family of cooperating effector genes conserved in Dothideomycetes and Sordariomycetes.Molecular plant pathology · 2023Article
- Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants.Molecular plant pathology · 2023Review
- "Late" effectors fromPlant direct · 2022Article
- A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins.PLoS pathogens · 2022Article
- Two independent approaches converge to the cloning of a new Leptosphaeria maculans avirulence effector gene, AvrLmS-Lep2.Molecular plant pathology · 2022Article
- Review
- A new avirulence gene of Leptosphaeria maculans, AvrLm14, identifies a resistance source in American broccoli (Brassica oleracea) genotypes.Molecular plant pathology · 2021Article
- Genome-wide mapping of histone modifications during axenic growth in two species of Leptosphaeria maculans showing contrasting genomic organization.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Analysis of the Oxidative Burst and Its Relevant Signaling Pathways inInternational journal of molecular sciences · 2021Article
- Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in theInternational journal of molecular sciences · 2021Article
- The Effect of Temperature on the Hypersensitive Response (HR) in thePlants (Basel, Switzerland) · 2021Article
- Dissection of Quantitative Blackleg Resistance Reveals Novel Variants of Resistance GeneFrontiers in plant science · 2021Article
- Recent Findings Unravel Genes and Genetic Factors UnderlyingInternational journal of molecular sciences · 2020Review
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12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassica napus (oilseed rape, canola) seedling resistance to Leptosphaeria maculans, the causal agent of blackleg (stem canker) disease, follows a gene-for-gene relationship. The avirulence genes AvrLmS and AvrLep2 were described to be perceived by the resistance genes RlmS and LepR2, respectively, present in B. napus 'Surpass 400'. Here we report cloning of AvrLmS and AvrLep2 using two independent methods. AvrLmS was cloned using combined in vitro crossing between avirulent and virulent isolates with sequencing of DNA bulks from avirulent or virulent progeny (bulked segregant sequencing). AvrLep2 was cloned using a biparental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach. Taking these two approaches independently, we found that AvrLmS and AvrLep2 are the same gene. Complementation of virulent isolates with this gene confirmed its role in inducing resistance on Surpass 400, Topas-LepR2, and an RlmS-line. The gene, renamed AvrLmS-Lep2, encodes a small cysteine-rich protein of unknown function with an N-terminal secretory signal peptide, which is a common feature of the majority of effectors from extracellular fungal plant pathogens. The AvrLmS-Lep2/LepR2 interaction phenotype was found to vary from a typical hypersensitive response through intermediate resistance sometimes towards susceptibility, depending on the inoculation conditions. AvrLmS-Lep2 was nevertheless sufficient to significantly slow the systemic growth of the pathogen and reduce the stem lesion size on plant genotypes with LepR2, indicating the potential efficiency of this resistance to control the disease in the field.
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