ArticlePLoS pathogens2022
Multiple knockout mutants reveal a high redundancy of phytotoxic compounds contributing to necrotrophic pathogenesis of Botrytis cinerea.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 107 citations in OpenAlex.
- SOBIR1 integrates pathogen recognition and hormonal metabolism in Nicotiana tabacum.Plant physiology · 2026Article
- The tomato cytoplasmic receptor-like kinase TPK09 mediates resistance to Botrytis cinerea and tolerance to light-induced stress.Plant physiology · 2026Article
- Host-Botrytis co-transcriptomics reveals finely tuned interactions with closely related legumes.G3 (Bethesda, Md.) · 2026Article
- CRISPR-Cas9-mediated targeted gene deletion inMicrobiology spectrum · 2026Article
- A multiplant transcriptomic atlas reveals conserved and lineage-specific defense architectures in response toProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Quiescence of postharvest pathogens: a fungal inhibition process or an immune response of the unripe host fruit?The New phytologist · 2026Review
- In vitro and in silico analysis of the fungicidal potential of secondary metabolites from a bacterial endophyte against Botrytis cinerea.BMC biotechnology · 2026Article
- Loss of virulence ofmBio · 2026Article
- Machine Learning- and AI-Driven QSAR Models for the Discovery of Novel Potential Fungicides: FAPI (Fungicide Targeting Acid Phosphatase Inhibition).Journal of agricultural and food chemistry · 2026Article
- Secreted Glycoside Hydrolase BcGH61 From Botrytis cinerea Induces Cell Death by the Apoplastic Location and Triggers Intracellular Immune Perception.Molecular plant pathology · 2026Article
- Transcription Factor PFB1 Is Required for the Botrytis cinerea Effector BcSCR1-Mediated Pathogenesis.Molecular plant pathology · 2026Article
- DNA Aptamers Targeting BcSOD1: A Novel Strategy for Controlling Botrytis cinerea in Sustainable Agriculture.Plant biotechnology journal · 2025Article
- Winning by Losing: Exploiting Modified Plant Susceptibility Genes to Counteract Necrotrophic Fungal Pathogens.Plant biotechnology journal · 2025Review
- Transformation of Alternaria dauci demonstrates the involvement of two polyketide synthase genes in aldaulactone production and fungal pathogenicity.Scientific reports · 2025Article
- Transcriptome Analysis ofMicroorganisms · 2025Article
- Learning from fungicide resistance: Evolutionary insights to guide RNAi-based control of fungal crop pathogens.Fungal biology reviews · 2025Article
- Mutation of PUB21 in tomato leads to reduced susceptibility to necrotrophic fungi.BMC plant biology · 2025Article
- Polygenic strategies for host-specific and general virulence of Botrytis cinerea across diverse eudicot hosts.Genetics · 2025Article
- Simply cut out - Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion inFrontiers in genome editing · 2025Article
- A glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum.Plant cell reports · 2024Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Botrytis cinerea is a major plant pathogen infecting more than 1400 plant species. During invasion, the fungus rapidly kills host cells, which is believed to be supported by induction of programmed plant cell death. To comprehensively evaluate the contributions of most of the currently known plant cell death inducing proteins (CDIPs) and metabolites for necrotrophic infection, an optimized CRISPR/Cas9 protocol was established which allowed to perform serial marker-free mutagenesis to generate multiple deletion mutants lacking up to 12 CDIPs. Whole genome sequencing of a 6x and 12x deletion mutant revealed a low number of off-target mutations which were unrelated to Cas9-mediated cleavage. Secretome analyses confirmed the loss of secreted proteins encoded by the deleted genes. Infection tests with the mutants revealed a successive decrease in virulence with increasing numbers of mutated genes, and varying effects of the knockouts on different host plants. Comparative analysis of mutants confirmed significant roles of two polygalacturonases (PG1, PG2) and the phytotoxic metabolites botrydial and botcinins for infection, but revealed no or only weak effects of deletion of the other CDIPs. Nicotiana benthamiana plants with mutated or silenced coreceptors of pattern recognition receptors, SOBIR1 and BAK1, showed similar susceptibility as control plants to infection by B. cinerea wild type and a 12x deletion mutant. These results raise doubts about a major role of manipulation of these plant defence regulators for B. cinerea infection. Despite the loss of most of the known phytotoxic compounds, the on planta secretomes of the multiple mutants retained substantial phytotoxic activity, proving that further, as yet unknown CDIPs contribute to necrosis and virulence. Our study has addressed for the first time systematically the functional redundancy of fungal virulence factors, and demonstrates that B. cinerea releases a highly redundant cocktail of proteins to achieve necrotrophic infection of a wide variety of host plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.