Evidence map›Paper›PMID 35239649›Full record

ArticlePLoS biology2022

The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast.

Sylvain Tollis, Jaspal Singh, Roger Palou, Yogitha Thattikota, Ghada Ghazal, Jasmin Coulombe-Huntington, Xiaojing Tang, Susan Moore, Deborah Blake, Eric Bonneil and 3 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. What programs the size of animal cells?Frontiers in cell and developmental biology · 2022
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Sylvain TollisInstitute of Biomedicine, University of Eastern Finland, Kuopio, Finland.ORCID 0000-0002-7301-4556
Jaspal SinghLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.ORCID 0000-0002-3223-910X
Roger PalouInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Yogitha ThattikotaInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Ghada GhazalInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Jasmin Coulombe-HuntingtonInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Xiaojing TangLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Susan MooreInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.ORCID 0000-0001-5401-2187
Deborah BlakeInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.ORCID 0000-0003-0727-4971
Eric BonneilInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.ORCID 0000-0002-4039-3131
Catherine A RoyerDepartment of Biological Sciences, Rensselaer Polytechnic Institute, Troy, New York, United States of America.
Pierre ThibaultInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Mike TyersInstitute for Research in Immunology and Cancer, University of Montréal, Montréal, Québec, Canada.
Institute for Research in Immunology and Cancer · CAMount Sinai Hospital · CARensselaer Polytechnic Institute · USUniversity of Eastern Finland · FI

Funding

BioGRID: An open resource for biological interactions and network analysisR01OD010929 · OD · SINAI HEALTH SYSTEM · PI KARA DOLINSKI, Mike Tyers · 2012 to 2026
$13.9M
CIHR FDN-167277NIH HHS R01 OD010929
6 · The paper itself

Abstract

Commitment to cell division at the end of G1 phase, termed Start in the budding yeast Saccharomyces cerevisiae, is strongly influenced by nutrient availability. To identify new dominant activators of Start that might operate under different nutrient conditions, we screened a genome-wide ORF overexpression library for genes that bypass a Start arrest caused by absence of the G1 cyclin Cln3 and the transcriptional activator Bck2. We recovered a hypothetical gene YLR053c, renamed NRS1 for Nitrogen-Responsive Start regulator 1, which encodes a poorly characterized 108 amino acid microprotein. Endogenous Nrs1 was nuclear-localized, restricted to poor nitrogen conditions, induced upon TORC1 inhibition, and cell cycle-regulated with a peak at Start. NRS1 interacted genetically with SWI4 and SWI6, which encode subunits of the main G1/S transcription factor complex SBF. Correspondingly, Nrs1 physically interacted with Swi4 and Swi6 and was localized to G1/S promoter DNA. Nrs1 exhibited inherent transactivation activity, and fusion of Nrs1 to the SBF inhibitor Whi5 was sufficient to suppress other Start defects. Nrs1 appears to be a recently evolved microprotein that rewires the G1/S transcriptional machinery under poor nitrogen conditions.

Indexed as

Gene Expression Regulation, FungalAmino Acid SequenceCell DivisionDNA-Binding ProteinsG1 PhaseImmunoblottingNitrogenProtein BindingRepressor ProteinsRNA-SeqSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidS PhaseTranscription FactorsDNA-Binding ProteinsNitrogenRepressor ProteinsSaccharomyces cerevisiae ProteinsSWI4 protein, S cerevisiaeSWI6 protein, S cerevisiaeTranscription FactorsWhi5 protein, S cerevisiae

Identifiers

PMID35239649
PMCPMC8893695
OpenAlexW4214875716

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.