ArticlePlant physiology2022
FvWRKY48 binds to the pectate lyase FvPLA promoter to control fruit softening in Fragaria vesca.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- WRKY transcription factors in horticultural crops: regulating quality traits and fruit stress responses.Planta · 2026Review
- QTL mapping for berry firmness and functional characterization of a prioritized candidate gene VvNAC83 in grape.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- FaRIF as a key regulator of strawberry fruit ripening: deciphering its targets and interaction networks.Horticulture research · 2026Article
- Transcription factor PpWRKY13 targets a cell wall modification gene in peach to regulate fruit softening.BMC plant biology · 2026Article
- The MabZIP5-MaMYB69 module cooperates with MaERF55 to modulate banana fruit ripening via cell wall degradation.Horticulture research · 2026Article
- Integrative multi-omics analysis unravels chitosan-mediated delay of mango fruit ripening through hormonal and metabolic reprogramming.Food chemistry. Molecular sciences · 2025Article
- Pectate LyasePlants (Basel, Switzerland) · 2025Article
- The role of pectate lyases in development and stress tolerance in plants.Molecular biology reports · 2025Review
- MdMAPK6-mediated phosphorylation of MdWRKY9 regulates apple fruit ripening through interaction with MdERF5L.Horticulture research · 2025Article
- The Ste20 Homologue FvM4K1 Regulates Organ Size via Hippo Signalling Pathway in Woodland Strawberry (Fragaria vesca).Plant biotechnology journal · 2025Article
- Transcriptomic analysis of Camellia japonica to scale insects infestation and functional characterization of pectin methylesterase gene CjPME28 and polygalacturonase gene CjPG1.Plant cell reports · 2025Article
- Article
- ERF100 regulated by ERF28 and NOR controls pectate lyase 7, modulating fig (Ficus carica L.) fruit softening.Plant biotechnology journal · 2025Article
- Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data.Scientific reports · 2025Article
- VvARF19 represses VvLBD13-mediated cell wall degradation to delay softening of grape berries.Horticulture research · 2025Article
- Allelic variation in an expansin, MdEXP-A1, contributes to flesh firmness at harvest in apples.Molecular horticulture · 2025Article
- Novel Targets for Fruit Conservation Strategies Revealed by Omics Studies: A Systematic Review and Meta-Analysis.International journal of food science · 2025Review
- MdWRKY31-MdNAC7 regulatory network: orchestrating fruit softening by modulating cell wall-modifying enzyme MdXTH2 in response to ethylene signalling.Plant biotechnology journal · 2024Article
- Members of WRKY Group III Transcription Factors Are Important in Mite Infestation in Strawberry (Plants (Basel, Switzerland) · 2024Article
- Cotton Pectate Lyase GhPEL48_Dt Promotes Fiber Initiation Mediated by Histone Acetylation.Plants (Basel, Switzerland) · 2024Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulatory mechanisms that link WRKY gene expression to fruit ripening are largely unknown. Using transgenic approaches, we showed that a WRKY gene from wild strawberry (Fragaria vesca), FvWRKY48, may be involved in fruit softening and ripening. We showed that FvWRKY48 is localized to the nucleus and that degradation of the pectin cell wall polymer homogalacturonan, which is present in the middle lamella and tricellular junction zones of the fruit, was greater in FvWRKY48-OE (overexpressing) fruits than in empty vector (EV)-transformed fruits and less substantial in FvWRKY48-RNAi (RNA interference) fruits. Transcriptomic analysis indicated that the expression of pectate lyase A (FvPLA) was significantly downregulated in the FvWRKY48-RNAi receptacle. We determined that FvWRKY48 bound to the FvPLA promoter via a W-box element through yeast one-hybrid, electrophoretic mobility shift, and chromatin immunoprecipitation quantitative polymerase chain reaction experiments, and β-glucosidase activity assays suggested that this binding promotes pectate lyase activity. In addition, softening and pectin degradation were more intense in FvPLA-OE fruit than in EV fruit, and the middle lamella and tricellular junction zones were denser in FvPLA-RNAi fruit than in EV fruit. We speculated that FvWRKY48 maybe increase the expression of FvPLA, resulting in pectin degradation and fruit softening.
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