ArticleScientific reports2022
Genotyping-in-Thousands by sequencing panel development and application for high-resolution monitoring of introgressive hybridization within sockeye salmon.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Article
- Kokanee-sockeye salmon hybridization predominantly leads to a resident life history with outcomes for a long-term reintroduction program.The Journal of heredity · 2026Article
- A Practical Framework for GT-Seq Panel Optimization.Molecular ecology resources · 2026Article
- GT-Seq Panel Development for Species Identification and Parentage Analysis of Closely Related Hybridising Scaphirhynchus Sturgeons.Molecular ecology resources · 2026Article
- Article
- A new genomic resource to enable standardized surveys of SNPs across the native range of brook trout (Salvelinus fontinalis).Molecular ecology resources · 2025Article
- Development of SNP Panels from Low-Coverage Whole Genome Sequencing (lcWGS) to Support Indigenous Fisheries for Three Salmonid Species in Northern Canada.Molecular ecology resources · 2025Article
- Development and validation of a GT-seq panel for genetic monitoring in a threatened species using minimally invasive sampling.Ecology and evolution · 2024Article
- Evaluating genotyping-in-thousands by sequencing as a genetic monitoring tool for a climate sentinel mammal using non-invasive and archival samples.Ecology and evolution · 2024Article
- A genotyping-in-thousands by sequencing panel to inform invasive deer management using noninvasive fecal and hair samples.Ecology and evolution · 2022Article
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Authors and funding
4 authors.
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Abstract
Stocking programs have been widely implemented to re-establish extirpated fish species to their historical ranges; when employed in species with complex life histories, such management activities should include careful consideration of resulting hybridization dynamics with resident stocks and corresponding outcomes on recovery initiatives. Genetic monitoring can be instrumental for quantifying the extent of introgression over time, however conventional markers typically have limited power for the identification of advanced hybrid classes, especially at the intra-specific level. Here, we demonstrate a workflow for developing, evaluating and deploying a Genotyping-in-Thousands by Sequencing (GT-seq) SNP panel with the power to detect advanced hybrid classes to assess the extent and trajectory of intra-specific hybridization, using the sockeye salmon (Oncorhynchus nerka) stocking program in Skaha Lake, British Columbia as a case study. Previous analyses detected significant levels of hybridization between the anadromous (sockeye) and freshwater resident (kokanee) forms of O. nerka, but were restricted to assigning individuals to pure-stock or "hybrid". Simulation analyses indicated our GT-seq panel had high accuracy, efficiency and power (> 94.5%) of assignment to pure-stock sockeye salmon/kokanee, F
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