ArticlePlanta2022
Hydroxycinnamoyltransferase and CYP98 in phenolic metabolism in the rosmarinic acid-producing hornwort Anthoceros agrestis.
Article in Planta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 8 citations in OpenAlex.
- Cytochrome P450 enzymes in the phenylpropanoid pathway: CYP73 and CYP98 candidate genes in Chara braunii, Anthoceros agrestis, Marchantia polymorpha and Physcomitrium patens.The Plant journal : for cell and molecular biology · 2026Article
- Completing the acylation landscape in plants: GDSL enzymes join the BAHD and SCPL acyltransferase families.Journal of experimental botany · 2026Review
- Identification and characterization of BAHD hydroxycinnamoyltransferases in the fern Neoblechnum brasiliense.The Plant journal : for cell and molecular biology · 2026Article
- Early occurrence of caffeoylshikimic acid esterase (CSE) activity in the hornwort Anthoceros agrestis.Planta · 2026Article
- The chromosome-scale assembly of the Salvia plebeia genome provides insight into the biosynthesis and regulation of rosmarinic acid.Plant biotechnology journal · 2025Article
- Phenolic metabolism in Sarcandra glabra is mediated by distinct BAHD hydroxycinnamoyltransferases.The Plant journal : for cell and molecular biology · 2025Article
- The evolution of plant responses underlying specialized metabolism in host-pathogen interactions.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Cytochromes P450 evolution in the plant terrestrialization context.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Promiscuity, a Driver of Plant Cytochrome P450 Evolution?Biomolecules · 2023Review
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
MAIN
conclusionAnthoceros agrestis hydroxycinnamoyltransferase accepts shikimic and 3-hydroxyanthranilic acids while hydroxycinnamoylester/amide 3-hydroxylase (CYP98A147) preferred p-coumaroyl-(3-hydroxy)anthranilic acid compared to the shikimic acid derivative. Alternative pathways towards rosmarinic acid have to be considered. Rosmarinic acid (RA) is a well-known ester of caffeic acid and 3,4-dihydroxyphenyllactic acid. In the search for enzymes involved in RA biosynthesis in the hornwort Anthoceros agrestis, the hydroxycinnamoyltransferase sequence with the highest similarity to rosmarinic acid synthase from Lamiaceae has been amplified and heterologously expressed in Escherichia coli. In parallel, the single cytochrome P450 sequence belonging to the CYP98 group in Anthoceros agrestis was isolated and expressed in Saccharomyces cerevisiae which did not result in protein formation. Codon optimization and co-expression with NADPH:cytochrome P450 reductase (CPR) from Coleus blumei resulted in the formation of active enzymes. Both, the hydroxycinnamoyltransferase and CYP98 were characterized with respect to their temperature and pH optimum as well as their substrate acceptance. The hydroxycinnamoyltransferase (AaHCT6) readily accepted p-coumaroyl- and caffeoyl-CoA with a slightly higher affinity towards p-coumaroyl-CoA. The best acceptor substrate was shikimic acid (K
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