ArticleGenetics2022
Novel genetic basis of resistance to Bt toxin Cry1Ac in Helicoverpa zea.
Article in Genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Effects of resistance to Bt proteins Cry1Ac and Vip3Aa on flight traits of Helicoverpa zea.Pest management science · 2026Article
- Editing the kinesin-12 gene affects responses to Bt toxin Cry1Ac in Helicoverpa zea.Scientific reports · 2025Article
- Evaluation of GS-omega/kappa-Hxtx-Hv1a and Bt toxins against Bt-resistant and -susceptible strains of Helicoverpa zea (Boddie) and Spodoptera frugiperda (J.E. Smith).Pest management science · 2025Article
- Article
- Genomic patterns of strain-specific genetic structure, linkage, and selection across fall armyworm populations.BMC genomics · 2025Article
- The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner).PloS one · 2025Article
- Polygenic response to selection by transgenic Bt-expressing crops in wild Helicoverpa zea and characterization of a major effect locus.BMC genomics · 2024Article
- Mismatch between lab-generated and field-evolved resistance to transgenic Bt crops inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rapid Adaptation and Interspecific Introgression in the North American Crop Pest Helicoverpa zea.Molecular biology and evolution · 2024Article
- A chromosome-level genome assembly of the soybean pod borer: insights into larval transcriptional response to transgenic soybean expressing the pesticidal Cry1Ac protein.BMC genomics · 2024Article
- Cross-pollination in seed-blended refuge and selection for Vip3A resistance in a lepidopteran pest as detected by genomic monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Chromosome-Scale Genome Assembly of a Helicoverpa zea Strain Resistant to Bacillus thuringiensis Cry1Ac Insecticidal Protein.Genome biology and evolution · 2023Article
- Comparison of Long-Read Methods for Sequencing and Assembly of Lepidopteran Pest Genomes.International journal of molecular sciences · 2022Article
- Knockout of ABC transporter gene ABCA2 confers resistance to Bt toxin Cry2Ab in Helicoverpa zea.Scientific reports · 2022Article
- Utility and challenges of using whole-genome resequencing to detect emerging insect and mite resistance in agroecosystems.Evolutionary applications · 2022Review
- Inheritance of Resistance to Cry1A.105 inInsects · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis have advanced pest management, but their benefits are diminished when pests evolve resistance. Elucidating the genetic basis of pest resistance to Bacillus thuringiensis toxins can improve resistance monitoring, resistance management, and the design of new insecticides. Here, we investigated the genetic basis of resistance to Bacillus thuringiensis toxin Cry1Ac in the lepidopteran Helicoverpa zea, one of the most damaging crop pests in the United States. To facilitate this research, we built the first chromosome-level genome assembly for this species, which has 31 chromosomes containing 375 Mb and 15,482 predicted proteins. Using a genome-wide association study, fine-scale mapping, and RNA-seq, we identified a 250-kb quantitative trait locus on chromosome 13 that was strongly associated with resistance in a strain of Helicoverpa zea that had been selected for resistance in the field and lab. The mutation in this quantitative trait locus contributed to but was not sufficient for resistance, which implies alleles in more than one gene contributed to resistance. This quantitative trait locus contains no genes with a previously reported role in resistance or susceptibility to Bacillus thuringiensis toxins. However, in resistant insects, this quantitative trait locus has a premature stop codon in a kinesin gene, which is a primary candidate as a mutation contributing to resistance. We found no changes in gene sequence or expression consistently associated with resistance for 11 genes previously implicated in lepidopteran resistance to Cry1Ac. Thus, the results reveal a novel and polygenic basis of resistance.
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